Nitrogen-containing compounds especially alkaloids are important medicinal ingredients in caulis dendrobii plants. Using solid-phase extraction coupled with liquid chromatography tandem mass spectrometry and multivariate data analysis methods, metabolic profiling of the nitrogen-containing compounds was established to distinguish Dendrobium huoshanense and Dendrobium officinale. Hundreds of nitrogen-containing compounds from the two caulis dendrobii were purified by the MCX cartridges. Some compounds were identified by high-resolution tandem mass spectrometry technology. Together with multivariate data analysis methods, comparative analysis of the metabolic profiling from two caulis dendrobii was conducted. A total of 133 nitrogen-containing compounds were identified, including amino acids, pyrrolidines, tropanes, pyrimidines, purines, indoles, piperidines, guanidines, quinolines, isoquinolines and terpenoids. Metabolic profiling analysis showed that the composition and contents of these chemical components were significantly different between D. huoshanense and D. officinale. Moreover, some components were species-specific, distributed in the two caulis dendrobii, such as pilosine, ternatusine, etc. Because alkaloids are mainly derived from amino acids via multistep biochemical reactions, the correlation analysis suggested that amino acids were partially associated with several types of components and significantly correlated with certain alkaloids. Arginine was extremely correlated with guanidines. Pyrimidines, purines and niacin-nicotinamide metabolic intermediates were associated with three independent networks. The results further enriched the chemical components currently identified from caulis dendrobii and provided a technical reference for detecting nitrogen-containing compounds in other medicinal plants.

中文翻译：

---

两个石D植物之间含氮代谢物的代谢组学分析。

含氮化合物，特别是生物碱是石ca植物中重要的药用成分。利用固相萃取结合液相色谱串联质谱法和多元数据分析方法，建立了含氮化合物的代谢谱图，以区分霍山石end和山石D。通过MCX纯化柱纯化了两个石uli中的数百种含氮化合物。通过高分辨率串联质谱技术鉴定了一些化合物。与多变量数据分析方法一起，对两种石uli的代谢谱进行了比较分析。总共鉴定出133种含氮化合物，包括氨基酸，吡咯烷，托烷，嘧啶，嘌呤，吲哚，哌啶，胍，喹啉，异喹啉和萜类化合物。代谢谱分析表明，霍山石and和山竹D.这些化学成分的组成和含量均存在显着差异。此外，某些成分是物种特异的，分布在两个石uli中，如油菜籽，松节油等。由于生物碱主要通过多步生化反应从氨基酸衍生而来，相关分析表明氨基酸部分与几种类型相关成分并与某些生物碱显着相关。精氨酸与胍极其相关。嘧啶，嘌呤和烟酸-烟酰胺代谢中间体与三个独立的网络相关。结果进一步丰富了目前从石ca中鉴定出的化学成分，并为检测其他药用植物中的含氮化合物提供了技术参考。