Abstract
Nitrogen-containing compounds especially alkaloids are important medicinal ingredients in caulis dendrobii plants. Using solid-phase extraction coupled with liquid chromatography tandem mass spectrometry and multivariate data analysis methods, metabolic profiling of the nitrogen-containing compounds was established to distinguish Dendrobium huoshanense and Dendrobium officinale. Hundreds of nitrogen-containing compounds from the two caulis dendrobii were purified by the MCX cartridges. Some compounds were identified by high-resolution tandem mass spectrometry technology. Together with multivariate data analysis methods, comparative analysis of the metabolic profiling from two caulis dendrobii was conducted. A total of 133 nitrogen-containing compounds were identified, including amino acids, pyrrolidines, tropanes, pyrimidines, purines, indoles, piperidines, guanidines, quinolines, isoquinolines and terpenoids. Metabolic profiling analysis showed that the composition and contents of these chemical components were significantly different between D. huoshanense and D. officinale. Moreover, some components were species-specific, distributed in the two caulis dendrobii, such as pilosine, ternatusine, etc. Because alkaloids are mainly derived from amino acids via multistep biochemical reactions, the correlation analysis suggested that amino acids were partially associated with several types of components and significantly correlated with certain alkaloids. Arginine was extremely correlated with guanidines. Pyrimidines, purines and niacin-nicotinamide metabolic intermediates were associated with three independent networks. The results further enriched the chemical components currently identified from caulis dendrobii and provided a technical reference for detecting nitrogen-containing compounds in other medicinal plants.
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Acknowledgements
Authors gratefully acknowledge National technical system of traditional Chinese medicine industry (Grant No. CARS-21), Collaborative innovation project of colleges and universities of Anhui (Grant Nos. GXXT-2019-043; GXXT-2019-049), Natural science research project of West Anhui University (Grant No. WXZR201911), Quality engineering project of West Anhui University (Grant No. wxxy2019080) and High-level Talents Research Initiation Funding Project (Grant No.WGKQ202001011) for the financial support.
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CS and CYJ conceived and designed the experiment. CS CYJ QJ performed the experiments. CS and CWC analyzed the data. YL and YPC contributed reagents/materials/analysis tools. CS wrote the paper.
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12298_2020_822_MOESM1_ESM.tif
Fig. S1 Liquid chromatogram of samples spiked with sarpagine. Sample spiked with standard (a) before SPE treatment and (b) after SPE treatment (TIF 567 kb)
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Fig. S2 Liquid chromatogram of samples spiked with sarpagine. Sample spiked with standard (a) before SPE treatment and (b) after SPE treatment (TIF 557 kb)
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Fig. S3 Liquid chromatogram of samples spiked with sarpagine. Sample spiked with standard (a) before SPE treatment and (b) after SPE treatment (TIF 567 kb)
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Fig. S4 Liquid chromatogram of samples spiked with reserpine. Sample spiked with standard (a) before SPE treatment and (b) after SPE treatment (TIF 455 kb)
12298_2020_822_MOESM5_ESM.tif
Fig. S5 Liquid chromatogram of samples spiked with reserpine. Sample spiked with standard (a) before SPE treatment and (b) after SPE treatment (TIF 495 kb)
12298_2020_822_MOESM6_ESM.tif
Fig. S6 Liquid chromatogram of samples spiked with reserpine. Sample spiked with standard (a) before SPE treatment and (b) after SPE treatment (TIF 447 kb)
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Song, C., Jiao, C., Jin, Q. et al. Metabolomics analysis of nitrogen-containing metabolites between two Dendrobium plants. Physiol Mol Biol Plants 26, 1425–1435 (2020). https://doi.org/10.1007/s12298-020-00822-1
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DOI: https://doi.org/10.1007/s12298-020-00822-1