Dendrobium Stardust ‘Fire Bird’ is an interspecific hybrid between D. unicum and D. Ukon ‘Arai’ and is known to be sterile and prevents further breeding. Chromosome doubling is an essential method to increase the size of plant organs, such as flowers, and restore fertility of interspecific hybrids, which enables further breeding for ornamental traits. To induce chromosome doubling, the protocorm-like bodies (PLBs) of a given variety were treated in vitro for 6 to 48 h with 10 to 160 mg L⁻¹ amiprofos-methyl (APM). Higher APM concentrations resulted in lower rates of survival of treated PLBs. Furthermore, subsequent growth of PLBs was hampered with prolonged APM treatment, although 10 and 20 mg L⁻¹ APM was optimal for doubling chromosomes. Plantlets with a swollen and shorter stem were observed after the APM treatment and these plants were also chromosome-doubled and became a distinctive morphological marker for the primary selection of chromosome-doubled plants. Chromosome-doubled plants of D. Stardust ‘Fire Bird’ will be utilized for the future breeding of unique Dendrobium cultivars.

石星尘“火鸟”是D之间的种间杂种。独角和d。Ukon'Arai'，众所周知是不育的，可防止进一步繁殖。染色体加倍是增加植物器官（例如花朵）的大小并恢复种间杂种的繁殖力的一种必不可少的方法，这使得能够进一步繁殖装饰性状。为了诱导染色体加倍，将给定变种的原球茎样体（PLB）在体外用10至160 mg L ^-1氨普罗磷甲基（APM）处理6至48小时。较高的APM浓度导致处理过的PLB的存活率较低。此外，长期的APM处理阻碍了PLB的后续生长，尽管10和20 mg L^-1 APM对于染色体加倍是最佳的。在APM处理后，观察到了具有肿胀且较短的茎的小植株，这些植株也被染色体加倍，并成为染色体加倍选择的独特形态标记。D的染色体倍增植物。星尘“火鸟”将用于未来独特石D兰品种的育种。