Skip to main content
SpringerLink
Log in

Efficient Chromosome Doubling of an Interspecific Hybrid Dendrobium Stardust ‘Fire Bird’ by Treatment of Amiprofos-methyl to Protocorm-Like Body

  • Biotechnology
  • Published:
In Vitro Cellular & Developmental Biology - Plant Aims and scope Submit manuscript

Abstract

Dendrobium Stardust ‘Fire Bird’ is an interspecific hybrid between D. unicum and D. Ukon ‘Arai’ and is known to be sterile and prevents further breeding. Chromosome doubling is an essential method to increase the size of plant organs, such as flowers, and restore fertility of interspecific hybrids, which enables further breeding for ornamental traits. To induce chromosome doubling, the protocorm-like bodies (PLBs) of a given variety were treated in vitro for 6 to 48 h with 10 to 160 mg L−1 amiprofos-methyl (APM). Higher APM concentrations resulted in lower rates of survival of treated PLBs. Furthermore, subsequent growth of PLBs was hampered with prolonged APM treatment, although 10 and 20 mg L−1 APM was optimal for doubling chromosomes. Plantlets with a swollen and shorter stem were observed after the APM treatment and these plants were also chromosome-doubled and became a distinctive morphological marker for the primary selection of chromosome-doubled plants. Chromosome-doubled plants of D. Stardust ‘Fire Bird’ will be utilized for the future breeding of unique Dendrobium cultivars.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1.
Figure 2.
Figure 3.
Figure 4.
Figure 5.
Figure 6.

Similar content being viewed by others

References

  • Abdoli M, Moieni A, Bad HN (2013) Morphological, physiological, cytological and phytochemical studies in diploid and colchicine-induced tetraploid plants of Echinacea purpurea (L.). Acta Physiol Plant 35:2075–2083

    Article  CAS  Google Scholar 

  • Adams PB (2011) Systematics of Dendrobiinae (Orchidaceae), with special reference to Australian taxa. Bot J Linn Soc 166:105–126

    Article  Google Scholar 

  • Allum JF, Bringloe DH, Roberts AV (2007) Chromosome doubling in a Rosa rugosa Thunb. hybrid by exposure of in vitro nodes to oryzalin: the effects of node length, oryzalin concentration and exposure time. Plant Cell Rep 26:1977–1984

    Article  CAS  Google Scholar 

  • Aoyama M, Kojima K, Kobayashi M (1994) Morphology of microspore in Phalaenopsis F1 hybrids. Kinki-Chugoku regional Agric Res 88:49–53 (Japanese)

  • Arditti J (2008) Micropropagation of orchids second edition Vol.1. Blackwell publishing, America

  • Baker ML, Baker CO (1996) Orchid species culture. Timber Press, Portland

    Google Scholar 

  • Barba-Gonzalez R, Lokker AC, Lim K-B, Ramanna MS, Van Tuyl JM (2004) Use of 2n gametes for the production of sexual polyploids from sterile Oriental × Asiatic hybrids of lilies (Lilium). Theor Appl Genet 109:1125–1132

    Article  CAS  Google Scholar 

  • Chen LL, Gao SL (2007) In vitro tetraploid induction and generation of tetraploids from mixoploids in Astragalus membranaceus. Sci Hortic 112:339–344

    Article  CAS  Google Scholar 

  • Chen WH, Tang CY, Kao YL (2009) Ploidy doubling by in vitro culture of excised protocorms or protocorm-like bodies in Phalaenopsis species. Plant Cell Tiss Org Cult 98:229–238

    Article  CAS  Google Scholar 

  • Chen WH, Tang CY, Lin TY, Weng YC, Kao YL (2011) Changes in the endopolyploidy pattern of different tissues in diploid and tetraploid Phalaenopsis aphrodite subsp. formosana (Orchidaceae). Plant Sci 181:31–38

    Article  CAS  Google Scholar 

  • De Mello e Silva M, Callegari J, Zanettini B (2000) Induction and identification of polyploids in Cattleya intermedia Lindl. (Orchidaceae) by in vitro techniques. Cienc Rural 30:105–111

    Article  Google Scholar 

  • Dhooghe E, Van Laere K, Eeckhaut T, Leus L, Van Huylenbroeck J (2011) Mitotic chromosome doubling of plant tissues in vitro. Plant Cell Tiss Org Cult 104:359–373

    Article  Google Scholar 

  • Fakhri Z, Mirzaghaderi G, Ahmadian S, Mason AS (2016) Unreduced gamete formation in wheat × Aegilops spp. hybrids is genotype specific and prevented by shared homologous subgenomes. Plant Cell Rep 35:1143–1154

    Article  CAS  Google Scholar 

  • Fukai S, Hasegawa A, Goi M (2002) Polysomaty in Cymbidium. HortScience 37:1088–1091

    Article  Google Scholar 

  • Ghanbari MA, Jowkar A, Salehi H, Zarei M (2019) Effects of polyploidization on petal characteristics and optical properties of Impatiens walleriana (Hook.). Plant Cell Tiss Org Cult 138:299–310

    Article  CAS  Google Scholar 

  • Grosso V, Farina A, Giorgi D, Nardi L, Diretto G, Lucretti S (2018) A high-throughput flow cytometry system for early screening of in vitro made polyploids in Dendrobium hybrids. Plant Cell Tiss Org Cult 132:57–70

    Article  Google Scholar 

  • Hossain MM, Sharma M, Pathak P (2013) In vitro propagation of Dendrobium aphyllum (Orchidaceae)—seed germination to flowering. Plant Biochem Biotechnol 22:157–167

    Article  Google Scholar 

  • Ishizaka H, Uematsu J (1994) Amphidiploids between Cyclamen persicum Mill. and C. hederifolim Aiton induced through colchicine treatment of ovules in vitro. Breed Sci 44:161–166

    Google Scholar 

  • Isshiki S, Taura T (2003) Fertility restoration of hybrids between Solanum melongena L. and S. aethiopicum L. Gilo Group by chromosome doubling and cytoplasmic effect on pollen fertility. Euphytica 134:195–201

    Article  Google Scholar 

  • Johansen B (1990) Incompatibility in Dendrobium (Orchidaceae). Bot J Linn Soc 103:165–196

    Article  Google Scholar 

  • Kamemoto H, Amore TD, Kuehnle AR (1996) Breeding Dendrobium orchid in Hawaii. University of Hawaii Press, Honolulu

    Google Scholar 

  • Khosravi P, Kermani MJ, Nematzadeh GA, Bihamta MR, Yokoya K (2008) Role of mitotic inhibitors and genotype on chromosome doubling of Rosa. Euphytica 160:267–275

    Article  CAS  Google Scholar 

  • Kim HH, Loh CS, Yeoh HH (2007) Early in vitro flowering and seed production in culture in Dendrobium Chao Praya Smile (Orchidaceae). Plant Cell Rep 26:2055–2062

    Article  Google Scholar 

  • Koehler S, Cabral JS, Whitten MW, Williams NH, Singer RB, Neubig K, Guerra M, Souza A. P, Carmo E (2008) Molecular phylogeny of the Neotropical genus Christensonella (Ochidaceae, Maxillarinae): species delimitation and insights into chromosome evolution. Ann Bot 102:490–507

  • Kreiner JM, Kron P, Husband BC (2017) Frequency and maintenance of unreduced gametes in natural plant populations: associations with reproductive mode, life history and genome size. New Phytol 214:879–889

    Article  CAS  Google Scholar 

  • Laskowska D, Berbeć A, Van Laere K, Kirov I, Czubacka A, Trojak-Goluch A (2015) Cytology and fertility of amphidiploid hybrids between Nicotiana wuttkei Clarkson et Symon and N. tabacum L. Euphytica 206:597–608

    Article  CAS  Google Scholar 

  • Lee YI, Chang FC, Chung MC (2011) Chromosome pairing affinities in interspecific hybrids reflect phylogenetic distances among lady's slipper orchids (Paphiopedilum). Ann Bot 108:113–121

    Article  Google Scholar 

  • Lee YI, Hsu ST, Yeung EC (2013) Orchid protocorm-like bodies are somatic embryos. Am J Bot 100:2121–2131

    Article  CAS  Google Scholar 

  • Lindsay GC, Hopping ME, O’Brien IEW (1994) Detection of protoplast-derived DNA tetraploid Lisianthus (Eustoma grandiflorum) by leaf and flower characteristics and by flow cytometry. Plant Cell Tiss Org Cult 38:53–55

    Article  Google Scholar 

  • Miguel TP, Leonhardt KW (2011) In vitro polyploid induction of orchids using oryzalin. Sci Hortic 130:314–319

    CAS  Google Scholar 

  • Nimura M, Kato J, Horaguchi H, Mii M, Sakai K, Katoh T (2006) Induction of fertile amphidiploids by artificial chromosome-doubling in interspecific hybrid between Dianthus caryophyllus L. and D. japonicus Thunb. Breed Sci 56:303–310

    Article  Google Scholar 

  • Nimura M, Kato J, Mii M, Ohishi K (2008) Cross-compatibility and the polyploidy of progenies in reciprocal backcrosses between diploid carnation (Dianthus caryophyllus L.) and its amphidiploid with Dianthus japonicus Thunb. Sci Hortic 115:183–189

    Article  Google Scholar 

  • Pinheiro F, Cafasso D, Cozzolino S, Scopece G (2015) Transitions between self-compatibility and self-incompatibility and the evolution of reproductive isolation in the large and diverse tropical genus Dendrobium (Orchidaceae). Ann Bot 116:457–467

    Article  CAS  Google Scholar 

  • Podwyszyńska M, Trzewik A, Marasek-Ciolakowska A (2018) In vitro polyploidisation of tulips (Tulipa gesneriana L.) phenotype assessment of tetraploids. Sci Hortic 242:155–163

    Article  Google Scholar 

  • Ramsey J (2007) Unreduced gametes and neopolyploids in natural populations of Achillea borealis (Asteraceae). Heredity 98:143–150

    Article  CAS  Google Scholar 

  • Sattler MC, Carvalho CR, Clarindo WR (2016) The polyploidy and its key role in plant breeding. Planta 243:281–296

    Article  CAS  Google Scholar 

  • Takamiya T, Wongsawad P, Sathapattayanon A, Tajima N, Suzuki S, Kitamura S, Shioda N, Handa T, Kitanaka S, Iijima H, Yukawa T (2014) Molecular phylogenetics and character evolution of morphologically diverse groups, Dendrobium section Dendrobium and allies. AoB PLANTS 6: plu045. https://doi.org/10.1093/aobpla/plu045

  • Takamura T, Miyajima I (1996) Colchicine induced tetraploids in yellow-flowered cyclamens and their characteristics. Sci Hortic 65:305–312

    Article  CAS  Google Scholar 

  • Teixeira da Silva JA, Singh N, Tanaka M (2006) Priming biotic factors for optimal protocorm-like body and callus induction in hybrid Cymbidium (Orchidaceae), and assessment of cytogenetic stability in regenerated plantlets. Plant Cell Tiss Org Cult 84:135–144

    Article  Google Scholar 

  • Tokuhara K, Mii M (1993) Micropropagation of Phalaenopsis and Doritaenopsis by culturing shoot tips of flower stalk buds. Plant Cell Rep 13:7–11

    Article  CAS  Google Scholar 

  • Vendrame WA, Carvalho VS, Dias JMM (2007) In vitro germination and seedling development of cryopreserved Dendrobium hybrid mature seeds. Sci Hortic 114:188–193

    Article  CAS  Google Scholar 

  • Wang ZH, Wang L, Ye Q (2009) High frequency early flowering from in vitro seedlings of Dendrobium nobile. Sci Hortic 122:328–331

    Article  CAS  Google Scholar 

  • Yamakawa M, Kishimoto T, Sato T, Saito T, Amano J, Otani M, Nakano M (2015) Chromosome doubling of a hybrid by spindle toxin treatments. Bull Facul Agric Niigata Univ 68:25–30, http://hdl.handle.net/10191/39812

  • Zhang X, Cao Q, Jia G (2017) A protocol for fertility restoration of F1 hybrid derived from Lilium × formolongi ‘Raizan 3’ × Oriental hybrid ‘Sorbonne’. Plant Cell Tiss Org Cult 129:375–386

    Article  Google Scholar 

  • Zhao H, Bughrara SS, Wang Y (2007) Cytology and pollen grain fertility in creeping bentgrass interspecific and intergeneric hybrids. Euphytica 156:227–235

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Graduate School of Horticulture, Chiba University, 648 Matsudo, Matsudo, Chiba, 271-8510, Japan

    Haruka Kondo, Wasana Phlaetita, Masahiro Mii, Shinji Kikuchi, Ayumi Deguchi & Kazumitsu Miyoshi

  2. Present Address: Department of Plant Science, Faculty of Natural Resources, Rajamangala University of Technology Isan, 744 Suranarai Road, Muang, Nakhon Ratchasima, 30000, Thailand

    Wasana Phlaetita

  3. Center for Environment, Health and Field Sciences, Chiba University, Kashiwanoha 6-2-1, Kashiwa, Chiba, 277-0882, Japan

    Masahiro Mii

Authors
  1. Haruka Kondo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Wasana Phlaetita
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Masahiro Mii
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Shinji Kikuchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ayumi Deguchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Kazumitsu Miyoshi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kazumitsu Miyoshi.

Additional information

Editor: Wagner Campos Otoni

Electronic supplementary material

ESM 1

(DOCX 49 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kondo, H., Phlaetita, W., Mii, M. et al. Efficient Chromosome Doubling of an Interspecific Hybrid Dendrobium Stardust ‘Fire Bird’ by Treatment of Amiprofos-methyl to Protocorm-Like Body. In Vitro Cell.Dev.Biol.-Plant 56, 738–749 (2020). https://doi.org/10.1007/s11627-020-10079-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11627-020-10079-8

Keywords

Search

Navigation