The effects of temperature in winter-spring (average 17.0 °C) and spring-summer (average 23.0 °C) seasons on the transmission and latent period of maize bushy stunt phytoplasma (MBS-phytoplasma) and corn stunt spiroplasma (CSS) (Spiroplasma kunkelii) caused by the corn leafhopper, Dalbulus maidis (DeLog and Wolcott) (Hemiptera: Cicadellidae) were evaluated in two experiments in a screenhouse in Sete Lagoas, Minas Gerais State, Brazil. Approximately 200 mollicute-free adults of D. maidis were confined on popcorn (Zea mays L. cv. IAC125) plants infected with spiroplasma and the same number of mollicute-free leafhoppers were confined on plants infected with phytoplasma. They remained on the source plants for a 6 d acquisition access period (AAP). Next, leafhoppers were fed healthy popcorn seedlings. Transmission tests were performed with one leafhopper per seedling for each mollicute, with 12 replicates, at 12, 20, 30, 40 d since the first day of AAP. In addition, at 40 d, one leafhopper with phytoplasma and another with spiroplasma were confined together on 24 popcorn seedlings. The inoculation access period (IAP) was 3 d. The temperature was recorded daily during the experimental periods. In Experiment 1, during the 40 d latent period, only 41.6% and 58.3% of plants, respectively, exposed to phytoplasma or to both mollicutes presented MBS-phytoplasma symptoms. In Experiment 2, during the 20 d latent period, some popcorn plants presented MBS-phytoplasma (16.7%) and CSS (33.3%) symptoms, whereas almost 100% of plants at the 30 and 40 d latent period presented disease symptoms. Plant symptoms and results from PCR tests indicated that MBS-phytoplasma was more tolerant to low temperatures than S. kunkelii.

冬春（平均17.0°C）和春夏（平均23.0°C）季节的温度对玉米丛生特技植原体（MBS-phytoplasma）和玉米特技螺旋体（CSS）（Spiroplasma）的传播和潜伏期的影响在巴西米纳斯吉拉斯州塞特拉各斯的一家筛房进行的两项实验中，对玉米叶蝉Dalbulus maidis（DeLog和Wolcott）（半翅目：Cicadellidae）引起的kunkelii进行了评估。约200名无D. maidis软体动物的成年人被限制在爆米花（Zea maysL.简历 IAC125）的植物被螺旋体感染，相同数量的无软体动物的叶蝉被限制在被植物体感染的植物上。他们在源工厂停留了6天的获取访问时间（AAP）。接下来，给叶蝉喂食健康的爆米花幼苗。从AAP的第一天开始，每摩尔每只幼苗用一只叶蝉进行传播试验，每12天重复一次，重复12次。此外，在第40天，将一只带有植物质体的叶蝉和另一只带有螺旋质体的叶蝉限定在24朵爆米花幼苗上。接种进入期（IAP）为3 d。在实验期间每天记录温度。在实验1中，在40天的潜伏期中，分别只有41.6％和58.3％的植物暴露于植物质原体或两种软体动物都呈现MBS-植物质原体症状。在实验2中，在20天潜伏期中，一些爆米花植物表现出MBS植原体（16.7％）和CSS（33.3％）症状，而在30天和40天潜伏期中几乎100％的植物表现出疾病症状。植物症状和PCR测试结果表明，MBS植物质体比低温更耐低温S.kunkelii。