Pesticide accumulation in agricultural soils is an environmental concern, often addressed through distinct bioremediation strategies. This study has tried to analyze various soil bioremediation options viz., biostimulation, bioaugmentation, and natural attenuation in terms of efficiency and the response of autochthonous microbial flora by using atrazine as a model contaminant. Soil mesocosms were established with 100 kg of soil simulating the field conditions. The soil previously exposed to the herbicide was used for the bioaugmentation strategy undertaken in this study. We have tried to analyze how the microbial community responds to a foreign compound, both in terms of taxonomic and functional capacities? To answer this, we have analyzed metagenome of the mesocosms at a time point when 90% atrazine was degraded. Bioaugmentation for bioremediation proved to be efficient with a DT90 value of 15.48 ± 0.79 days, in comparison to the natural attenuation where the DT90 value was observed to be 41.20 ± 1.95 days. Metagenomic analysis revealed the abundance of orders Erysipelotrichales, Selemonadales, Clostridiales, and Thermoanaerobacterales exclusively in SBS mesocosm. Besides Pseudomonas, bacterial genera such as Achromobacter, Xanthomonas, Stenotrophomonas, and Cupriavidus have emerged as the dominant members in various bioremediation strategies tested in this study. Inclusive results suggest that inherent microbial flora adjust their community and metabolic machinery upon exposure to the pollutant. The site under pollutant stress showed efficient microbial communities to bio-remediate the newly polluted terrestrial ecologies in relatively less time and by economic means.

中文翻译：

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通过宏基因组学分析阿特拉津生物修复及其对土壤微生物多样性的影响。

农业土壤中的农药积累是一个环境问题，通常通过不同的生物修复策略来解决。本研究试图通过使用莠去津作为模型污染物，分析各种土壤生物修复方案，即生物刺激、生物强化和自然衰减，以提高效率和本地微生物菌群的响应。用 100 公斤土壤模拟田间条件建立了土壤中观。先前暴露于除草剂的土壤用于本研究中进行的生物强化策略。我们试图分析微生物群落如何在分类和功能方面对外来化合物做出反应？为了回答这个问题，我们在 90% 阿特拉津降解的时间点分析了中宇宙的宏基因组。与 DT90 值为 41.20 ± 1.95 天的自然衰减相比，生物修复的生物强化被证明是有效的，其 DT90 值为 15.48 ± 0.79 天。宏基因组分析揭示了 Erysipelotrichales、Selemonadales、Clostridiales 和 Thermoanaerobacterales 的丰度，它们仅存在于 SBS 中胚层中。除了假单胞菌属、无色杆菌属、黄单胞菌属、窄养单胞菌属和铜绿单胞菌属等细菌属已成为本研究中测试的各种生物修复策略的主要成员。包容性结果表明，固有微生物菌群在暴露于污染物后会调整其群落和代谢机制。处于污染物压力下的场地显示出有效的微生物群落，可以在相对较短的时间内通过经济手段对新污染的陆地生态进行生物修复。