Most of previous works studied the bioactivity of tertiary soda-lime-borate as in vitro method, but, there have been no previous studies investigated the in vivo compatibility of such glass. This work was mainly aimed at in vivo assessment of bone formation of tertiary soda-lime-borate bioactive glass doped with Sr. the glass composition was based on 60 B₂O₃–20 Na₂O–(20-x) CaO–xSrO (wt%), where, x = 0 and 10 wt% (samples encoded B0 and BS, respectively). The in vivo test was conducted in femoral bone defect of Sprague-Dawley adult male rats after 3 and 6 weeks post-surgery using the histological analyses and bone formation markers (alkaline phosphatase (ALP) and osteocalcin (OCN)). Moreover, the possible systemic toxicity was studied using different biochemical analyses (alanine transaminase, aspartate transaminase, urea and creatinine). The result of bone markers showed that serum OCN level increased in rat implanted with sample B0 than that observed in sample BS at 3 and 6 weeks post-implantation, while this finding was reversed in ALP activity. In vivo bioactivity test showed that implantation of all borate glasses did not demonstrate local or general complications in all rats, and they exhibited nearly complete bone mineralization. However, BS glass was formed more new bone % than B0 one in long implantation time. In conclusion, the synthesized bioactive borate glasses were safe materials, and introducing of Sr in the glass was enhanced formation of new bone throughout long time of implantation. Accordingly, this glass can be used as a potential substitute for bone regenerative materials and a hosting for strontium ions delivery.

先前的大多数工作都以体外方法研究了叔碳酸钙-硼酸盐的生物活性，但是，以前没有研究对这种玻璃的体内相容性进行研究。这项工作的主要目的是在体内评估掺有Sr的叔碳酸钙-硼酸盐生物活性玻璃的骨形成。该玻璃成分基于60 B ₂ O ₃ –20 Na ₂ O–（20- x）CaO– x SrO（wt％），其中，x = 0和10 wt％（样本分别编码为B0和BS）。使用组织学分析和骨形成标记物（碱性磷酸酶（ALP）和骨钙蛋白（OCN）），在手术后3周和6周对Sprague-Dawley成年雄性大鼠的股骨缺损进行体内测试。此外，使用不同的生化分析（丙氨酸转氨酶，天冬氨酸转氨酶，尿素和肌酐）研究了可能的全身毒性。骨标记物的结果显示，在植入B0的大鼠中，血清OCN水平比在植入后3周和6周的BS中所观察到的升高，而这一发现在ALP活性上却相反。体内生物活性测试表明，所有硼酸盐玻璃的植入均未在所有大鼠中显示出局部或一般并发症，并且它们表现出几乎完全的骨矿化。但是，在较长的植入时间内，BS玻璃比B0形成了更多的新骨％。总之，合成的生物活性硼酸盐玻璃是安全的材料，并且在玻璃中引入Sr可以在整个长期植入过程中增强新骨的形成。因此，该玻璃可用作骨再生材料的潜在替代物和锶离子传递的基质。