Omega-3 fatty acids have immense health benefits. But developing omega-3 fatty acids fortified food is a challenging task. The aim of this study was to evaluate possible antioxidant potential of hydro ethanol extract of peels of five selected varieties of potatoes (Kufri Chipsona-1, Kufri Chipsona-3, Kufri Chipsona-4, Kufri Chandramukhi and Kufri Jyoti) in various in vitro models and in lipid substrate enriched with omega-3 fatty acids. In vitro antioxidant potential was evaluated by DPPH radical scavenging, β-carotene linoleic acid bleaching and Fe²⁺ ion chelating methods. Anti-lipid peroxidation potential was evaluated in omega-3 fatty acids-enriched bulk oil and oil-in-water emulsion model systems using peroxide value (PV), p-anisidine value (p-AV) and TOTOX value as indicators of oxidation. Chemical characterization of compounds was performed by LC–ESI–MS/MS analysis and cytotoxic potential was evaluated by brine shrimp lethality assay. Among the peel extracts tested, Kufri Chipsona-3 potato peel extract was found to be most effective antioxidant in both the test model systems followed by Kufri Jyoti and Kufri Chandramukhi. Chemical analysis revealed the presence of phenolic acids, flavonoids and anthocyanidins as major constituents of these three potent extracts. These potent potato peel extracts were also found to be non-toxic at recommended dosage level (LC₅₀ > 1000 µg/ml). The results provide evidence that peel extract of these three potato varieties, especially Kufri Chipsona-3, may serve as a potential source of natural antioxidants for retarding oxidation of omega-3 fatty acids-enriched food supplement which subsequently may help in developing omega-3 fatty acids fortified foods.

中文翻译：

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在不同的体外模型和富含omega-3脂肪酸的脂质底物中，对某些选定品种的马铃薯皮的水乙醇提取物进行化学分析，细胞毒性研究和抗氧化能力评估

Omega-3脂肪酸具有巨大的健康益处。但是开发omega-3脂肪酸强化食品是一项艰巨的任务。这项研究的目的是评估在各种体外模型中五个选定品种的马铃薯（Kufri Chipsona- 1 ，Kufri Chipsona- 3 ，Kufri Chipsona- 4 ，Kufri Chandramukhi和Kufri Jyoti）的果皮中水乙醇提取物可能具有的抗氧化潜力。和富含omega-3脂肪酸的脂质底物中。通过DPPH自由基清除，β-胡萝卜素亚油酸漂白和Fe ²⁺评估体外抗氧化能力离子螯合方法。使用过氧化物值（PV），对氨基苯甲酸值（p -AV）和TOTOX值作为氧化指标，在富含omega-3脂肪酸的散装油和水包油乳液模型系统中评估了抗脂质过氧化的潜力。通过LC–ESI–MS / MS分析对化合物进行化学表征，并通过盐水虾致死率分析评估其细胞毒性潜力。在测试的果皮提取物中，Kufri Chipsona- 3马铃薯果皮提取物在两种测试模型系统中均被发现是最有效的抗氧化剂，其次是Kufri Jyoti和Kufri Chandramukhi。化学分析表明，酚酸，类黄酮和花青素是这三种有效提取物的主要成分。在推荐的剂量水平（LC ₅₀  > 1000 µg / ml）下，也发现这些有效的马铃薯皮提取物无毒。结果提供了证据，这三个马铃薯品种的果皮提取物，特别是Kufri Chipsona- 3，可以作为天然抗氧化剂的潜在来源，以延迟富含omega-3脂肪酸的食品补充剂的氧化，从而有助于开发omega-3脂肪酸强化食品。