Abstract
Omega-3 fatty acids have immense health benefits. But developing omega-3 fatty acids fortified food is a challenging task. The aim of this study was to evaluate possible antioxidant potential of hydro ethanol extract of peels of five selected varieties of potatoes (Kufri Chipsona-1, Kufri Chipsona-3, Kufri Chipsona-4, Kufri Chandramukhi and Kufri Jyoti) in various in vitro models and in lipid substrate enriched with omega-3 fatty acids. In vitro antioxidant potential was evaluated by DPPH radical scavenging, β-carotene linoleic acid bleaching and Fe2+ ion chelating methods. Anti-lipid peroxidation potential was evaluated in omega-3 fatty acids-enriched bulk oil and oil-in-water emulsion model systems using peroxide value (PV), p-anisidine value (p-AV) and TOTOX value as indicators of oxidation. Chemical characterization of compounds was performed by LC–ESI–MS/MS analysis and cytotoxic potential was evaluated by brine shrimp lethality assay. Among the peel extracts tested, Kufri Chipsona-3 potato peel extract was found to be most effective antioxidant in both the test model systems followed by Kufri Jyoti and Kufri Chandramukhi. Chemical analysis revealed the presence of phenolic acids, flavonoids and anthocyanidins as major constituents of these three potent extracts. These potent potato peel extracts were also found to be non-toxic at recommended dosage level (LC50 > 1000 µg/ml). The results provide evidence that peel extract of these three potato varieties, especially Kufri Chipsona-3, may serve as a potential source of natural antioxidants for retarding oxidation of omega-3 fatty acids-enriched food supplement which subsequently may help in developing omega-3 fatty acids fortified foods.
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References
Lunn J, Theobald H (2006) The health effects of dietary unsaturated fatty acids. Nutr Bull 31:78–224
Tehrany EB, Jacquot M, Gaiani C, Imran M, Desobry S, Linder M (2012) Beneficial effects and oxidative stability of omega-3 long-chain polyunsaturated fatty acids. Trends Food Sci Technol 25:24–33
Schmit G, Ecker J (2008) The opposing effects of n-3 and n-6 fatty acids. Prog Lipid Res 47(2):147–155
Huerta-Yepe S, Tirado-Rodrigue AB, Hannkinsona O (2016) Role of diets rich in omega-3 fatty acids. Bol Med del Hosp Inf de Mex 73(6):446–456
Simopoulos AP (2008) The importance of the omega-6/omega-3 fatty acid ratio in cardiovascular disease and other chronic diseases. Exp Biol Med J 233(6):674–688
Yepez SH, Ana B, Rodriguez T, Hankinson O (2016) Role of diets rich in omega-3 and omega-6 in the development of cancer. Bol Med del Hosp Inf de Mex 73(6):446–456
Jacobsen C, Sorensen AD, Nielsen N (2013) Stabilization of omega-3 oils and enriched foods using antioxidants. In: Food enrichment with omega-3 fatty acids. Woodhead Publishing Series in Food Science, Technology and Nutrition, pp 130–149. https://doi.org/10.1533/97805709863.2.130
Jacobsen C (2010) Enrichment of foods with omega-3 fatty acids: a multidisciplinary challenge. Ann N Y Acad Sci 1190:141–150
Habeebullah SFK, Nielsen NS, Jacobsen C (2010) Antioxidant activity of potato peel extracts in a fish-rapeseed oil mixture and in oil-in-water emulsions. J Am Oil Chem Soc 87(11):1319–1332
Rice-Evans C, Miller N, Paganga G (1997) Antioxidant properties of phenolic compounds. Trends Plant Sci 2(4):152–159
Craft BD, Adrian L, Amarowicz KR (2012) Phenol-based antioxidants and the in vitro methods used for their assessment. Comp Rev Food Sci Food Safety 11(2):148–172
Anwar H, Hussain G, Mustafa I (2018) Antioxidants from natural sources. In: Emad Shalaby (ed) Antioxidants in foods and its applications. IntechOpen. https://doi.org/10.5772/intechopen.72008
Akyol H, Riciputi Y, Capanoglu E, Caboni MF, Verardo V (2016) Phenolic compounds in the potato and its byproducts: an overview. Int J Mol Sci 17(6):835
Kanatt S, Chander R, Radhakrishna P, Sharma A (2005) Potato peel extract a natural antioxidant for retarding lipid peroxidation in radiation processed lamb meat. J Agric Food Chem 53:1499–1504
Mohdaly A, Sarhan M, Smetanska I, Mahmoud A (2010) Antioxidant properties of various solvent extracts of potato peel, sugar beet pulp and sesame cake. J Sci Food Agric 90:218–222
Amado I, Franco D, Sánchez M, Zapata C, Vazquez J (2014) Optimisation of antioxidant extraction from Solanum tuberosum potato peel waste by surface response methodology. Food Chem 165:290–299
Franco D, Pateiro M, Amado IR, Pedrouse ML, Zapata C, Vaquez JA, Lorenzo JM (2016) Antioxidant activity of potato (Solanum tuberosum) peel extracts to inhibit Soybean oil oxidation. Eur J Lipid Sci Technol 118(12):1891–1902
Mohdaly A, Hassanien M, Mahmoud A, Sarhan M, Smetanska I (2013) Phenolics extracted from potato, sugar beet, and sesame processing by-products. Int J Food Prop 16:1148–1168
Habeebullah SFK, Grejsen HD, Jacobsen C (2012) Potato peel extract as a natural antioxidant in chilled storage of minced horse mackerel (Trachurus trachurus): effect on lipid and protein oxidation. Food Chem 131:843–851
Albishi T, John J, Al-Khalifa A, Shahidi F (2013) Phenolic content and antioxidant activities of selected potato varieties and their processing by-products. J Funct Foods 5:590–600
Abu-Reidah IM, Arraez-Roman D, Segura-Carretero A, Fernandez-Gutierrez A (2013) Profiling of phenolic and other polar constituents from hydromethanolic extract of watermelon (Citrullus lanatus) by means of accuratemass spectrometry (HPLC–ESI–QTOF–MS). Food Res Intern 51:354–362
Wang M, Li J, Rangarajan M, Shao Y, LaVoie EJ, Huang T (1998) Antioxidative phenolic compounds from sage (Salvia officinalis). J Agric Food Chem 46:4869–4873
Velioglu VS, Mazza G, Gao L, Oomah BD (1998) Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J Agric Food Chem 46:4113–4117
Dinis TCP, Madeira VMC, Almeida LM (1994) Action of phenolic derivatives (acetaminophen, salicylate, and 5-aminosalicylate) as inhibitors of membrane lipid. Arch Biochem Biophys 315(1):161–169
Yamamoto Y, Misawa R (2018) Effect of emulsifier concentration on the oxidation of an O/W emulsion prepared from canola oil. Food Nutr Sci 9:683–692
AOCS (American Oil Chemists’ Society) (1998) Official method cd 8-53, Peroxide value. In: Firestone D (ed) Official methods and recommended practices of the american oil chemists’ society, 5th edn. AOCS, Chamaign
Reddy KJ, Jayathilakan KK, Pandey MC, Radhakrishna KK (2013) Evaluation of the physico-chemical stability of rice-bran oil and its blends for the development of functional meat products. Int J Food Sci Nutr 2(2):46–53
Poiana MA, Moigradean D, Raba D, Alda LM, Popa M (2010) The effect of long-term frozen storage on the nutraceutical compounds, antioxidant properties and color indices of different kinds of berries. J Food Agric Environ 8(1):54–58
Meyer BN, Ferrigni NR, Putnam JE, Jacobsen LB, Nichols DJ, McLaughlin JL (1982) Brine shrimp: a convenient general bioassay for active plant constituents. Planta Med 45:31–34
Afanasev IB, Dcrozhko AI, Brodskii AV, Kostyuk VA, Potapovitch AI (1989) Chelating and free radical scavenging mechanisms of inhibitory action of rutin and quercetin in lipid peroxidation. Biochem Pharmacol 38:1763–1769
Minatel IO, Borges C, Ferreira MI, Gomez GH, Chen O, Pace G, Lima G (2017) Phenolic compounds: functional properties, impact of processing and bioavailability. In: Hernandez M (ed) Phenolic compounds-biological activity, pp 1–24. https://doi.org/10.5772/intechopen.66368
Kedare SB, Singh RP (2011) Genesis and development of DPPH method of antioxidant assay. J Food Sci Technol 48(4):412–422
Frankel EN (1998) Hydroperoxide formation. In: Frankel EN (ed) Lipid oxidation. The Oily Press, Dundee, pp 23–41
Kulisic T, Radonic A, Katalinic V, Milos M (2004) Use of different methods for testing antioxidative activity of oregano essential oil. Food Chem 85:633–640
Hippeli S, Elstner EF (1999) Transition metal ion-catalyzed oxygen activation during pathogenic processes. FEBS Lett 443:1–7
Pazos M, Gallardo JM, Torres JL, Medina I (2005) Activity of grape polyphenols as inhibitors of the oxidation of fish lipids and fish muscle. Food Chem 92:547–557
Kumar S, Singh A, Bajpai V, Srivastava M, Singh BP, Kumar B (2016) Structural characterization of monoterpene indole alkaloids in ethanolic extracts of Rauwolfia species by liquid chromatography with quadrupole time-of-flight mass spectrometry. J Pharm Anal 6:363–373
Reddy CVK, Sreeramulu D, Raghunath M (2010) Antioxidant activity of fresh and dry fruits commonly consumed in India. Food Res Int 43:285–288
Someya S, Yoshiki Y, Okubo K (2002) Antioxidant compounds from bananas (Musa cavendish). Food Chem 79:351–354. https://doi.org/10.1016/S0308-8146(02)00186-3
Sarawong C, Schoenlechner R, Sekiguchi K, Berghofer E, Ng PKW (2014) Effect of extrusion cooking on the physicochemical properties, resistant starch, phenolic content and anti-oxidant capacities of green banana flour. Food Chem 143:33–39
Ling Y, Zhang Q, Zhiwen Fu, Lingling Xu (2015) Identification and structural elucidation of steroidal saponins from the root of Paris polyphylla by HPLC-ESI–QTOF-MS/MS. Nat Prod Res 29:1798–1803
Chen XF, Wu HT, Tan G, Hy ZY, Chai YF (2011) Liquid chromatography coupled with time-of-flight and iron trap mass spectrometry for qualitative analysis of herbal medicines. J pharma Anal. 1(4):235–245
Acknowledgements
Authors are thankful to the Dean of Studies, Indian Statistical Institute, Kolkata, India for providing funding in order to pursue research work. Authors also wish to acknowledge the Head, SAIF, Central Drug Research Institute, Lucknow, India for LC–ESI–MS/MS facility and cooperation.
Funding
Dean of studies, Indian Statistical Institute, Kolkata, India has provided funding to carry out the research work. [Internally funded project; Project number: 5495C (2018-2019)].
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Bhattacharya, A., Purkait, S., Bag, A. et al. Chemical profiling, cytotoxicity study and assessment of antioxidant potential of hydro-ethanol extract of peels of some selected varieties of potato in various in vitro models and in lipid substrate enriched with omega-3 fatty acids. Eur Food Res Technol 246, 1469–1482 (2020). https://doi.org/10.1007/s00217-020-03504-0
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DOI: https://doi.org/10.1007/s00217-020-03504-0