Pot marigold and tickseed are ornamental plants with many medicinal and cosmetic uses and for landscape, respectively. During a survey in 2018, phyllody symptoms were observed in high percentages in these plants in some regions of the Razavi Khorasan province (northeastern Iran). Total DNA was extracted from symptomatic and asymptomatic plants and polymerase chain reaction was carried on using universal phytoplasma primer pairs P1/P7 and nested primer pairs R16F2n/R16R2. The nested amplification of 1200-bp fragments confirmed the presence of phytoplasmas only in the symptomatic plants. BLAST search, phylogenetic analysis, and virtual RFLP patterns of cloned amplicons allowed to classify the pot marigold phyllody phytoplasma in the 16SrVI-A subgroup while tickseed phyllody was enclosed in the 16SrIX-I subgroup. This is the first report of the association of a 16SrVI phytoplasma with pot marigold phyllody in Iran and of the phytoplasma presence in tickseed.

中文翻译：

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在伊朗东北部的万寿菊和壁虱植物中检测到16SrVI和16SrIX植物质群。

万寿菊和凤眼兰分别是观赏植物，具有许多药用和美容用途，并用于园林。在2018年的一项调查中，在Razavi Khorasan省（伊朗东北）的某些地区，这些植物的叶状体症状百分率很高。从有症状和无症状植物中提取总DNA，并使用通用的植物原质对P1 / P7和嵌套引物对R16F2n / R16R2进行聚合酶链反应。1200 bp片段的嵌套扩增证实仅在有症状的植物中存在植物质原体。克隆扩增子的BLAST搜索，系统发生分析和虚拟RFLP模式可对16SrVI-A子群中的盆栽万寿菊叶状植物原质进行分类，而tick豆状的叶状体被封闭在16SrIX-I子组中。