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Detection of 16SrVI and 16SrIX phytoplasma groups in pot marigold and tickseed plants in northeastern Iran

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Abstract

Pot marigold and tickseed are ornamental plants with many medicinal and cosmetic uses and for landscape, respectively. During a survey in 2018, phyllody symptoms were observed in high percentages in these plants in some regions of the Razavi Khorasan province (northeastern Iran). Total DNA was extracted from symptomatic and asymptomatic plants and polymerase chain reaction was carried on using universal phytoplasma primer pairs P1/P7 and nested primer pairs R16F2n/R16R2. The nested amplification of 1200-bp fragments confirmed the presence of phytoplasmas only in the symptomatic plants. BLAST search, phylogenetic analysis, and virtual RFLP patterns of cloned amplicons allowed to classify the pot marigold phyllody phytoplasma in the 16SrVI-A subgroup while tickseed phyllody was enclosed in the 16SrIX-I subgroup. This is the first report of the association of a 16SrVI phytoplasma with pot marigold phyllody in Iran and of the phytoplasma presence in tickseed.

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Acknowledgments

We thank Dr. Narjes Azizi from Forest and Rangeland Department, Khorasan Razavi Agricultural and Natural Resource Research and Education center, AREEO, Mashhad, Iran, for identification of plant species.

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Authors and Affiliations

  1. Plant Protection Research Department, Khorasan Razavi Agricultural and Natural Resources Research and Education Center, AREEO, Mashhad, Iran

    Sara Gharouni-Kardani

  2. Department of Plant Protection, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

    Mahnaz Ashnayi

  3. Department of Agricultural and Food Sciences, Alma Mater Studiorum University of Bologna, Bologna, Italy

    Assunta Bertaccini

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  1. Sara Gharouni-Kardani
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  2. Mahnaz Ashnayi
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  3. Assunta Bertaccini
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Correspondence to Sara Gharouni-Kardani.

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Gharouni-Kardani, S., Ashnayi, M. & Bertaccini, A. Detection of 16SrVI and 16SrIX phytoplasma groups in pot marigold and tickseed plants in northeastern Iran. Folia Microbiol 65, 697–703 (2020). https://doi.org/10.1007/s12223-020-00772-x

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