Lactobacillus plantarum EM is a probiotic strain with antimicrobial activity, cholesterol-lowering effects, and tolerance to acid and bile. To understand the genetic basis of the probiotic characteristics of this strain, genome sequencing and probiotic-related genetic analysis were performed. The genomic characteristics of L. plantarum EM were confirmed by comparative genomic analysis with 41 probiotic lactic acid bacteria, including 10 L. plantarum strains. L. plantarum EM was shown to contain a circular chromosome of 3,184,808 bp and eight plasmids with various lengths from 5,027 to 76,369 bp. The L. plantarum EM genome had a total of 3560 protein-coding genes, including probiotic-related genes, such as tolerance to acid and bile, temperature stress, and oxidative stress. Comparative genomic analysis showed that L. plantarum EM contained plantaricin and bovicin gene clusters, which are related to antimicrobial activity, and five bile salt hydrolase genes related to serum cholesterol-lowering effects. The genomic analysis confirmed the probiotic properties of L. plantarum EM, and our results indicated that this strain has potential application for use as an industrially important probiotic.

中文翻译：

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植物乳杆菌 EM 的完整基因组序列，一种具有降低胆固醇作用和抗菌活性的推定益生菌菌株

植物乳杆菌 EM 是一种益生菌菌株，具有抗菌活性、降低胆固醇的作用以及对酸和胆汁的耐受性。为了了解该菌株益生菌特性的遗传基础，进行了基因组测序和益生菌相关遗传分析。植物乳杆菌 EM 的基因组特征通过与 41 种益生菌乳酸菌（包括 10 种植物乳杆菌菌株）的比较基因组分析得到证实。植物乳杆菌 EM 显示含有 3,184,808 bp 的环状染色体和 8 个长度从 5,027 到 76,369 bp 不等的质粒。植物乳杆菌 EM 基因组共有 3560 个蛋白质编码基因，包括益生菌相关基因，如耐酸胆、温度胁迫和氧化胁迫等。比较基因组分析表明，L. Plantarum EM 含有与抗菌活性相关的植物素和牛黄素基因簇，以及与降低血清胆固醇作用相关的五个胆汁盐水解酶基因。基因组分析证实了植物乳杆菌 EM 的益生菌特性，我们的结果表明该菌株具有用作工业重要益生菌的潜在应用。