Hippophae rhamnoides L. provides an enormous range of medicinal and nutritional benefits. The significant abilities of this plant to survive in Himalayan high altitudes enticed our study to investigate its rhizosphere. Seventeen rhizobacterial strains were isolated from the rhizospheric soil and plant root nodules, belonging to genus Frankia, Azorhizobium, Bacillus, Paenibacillus, Brevibacillus and Pseudomonas, as identified by 16SrRNA sequencing. This varying bacterial population was further examined for the presence of root degrading enzymes pectinase and cellulase, which enable them to intrude the plant roots. Based on the growth and substrate utilization by these rhizobacteria on pectinase screening agar medium and Mandels and Reese agar medium, all the seventeen strains were identified as pectinase and cellulase producing rhizobacteria. The quantitative analysis by DNS method demonstrated varying enzyme activities, spot-lighting the physiological variation in the microbiome. The divergence in the enzyme activities shown by all the strains was analysed statistically, using the software ASSISTAT.

中文翻译：

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沙棘根际细菌表现出多样化的纤维素酶和果胶酶活性。

沙棘具有广泛的药用和营养价值。这种植物在喜马拉雅高海拔地区生存的显着能力促使我们研究其根际。从根际土壤和植物根瘤中分离得到17株根际细菌，经16SrRNA测序鉴定，分别属于Frankia属、Azorhizobium属、Bacillus属、Paenibacillus属、Brevibacillus属和Pseudomonas属。进一步检查了这种不同的细菌种群是否存在根部降解酶果胶酶和纤维素酶，这使得它们能够侵入植物根部。根据这些根际细菌在果胶酶筛选琼脂培养基和曼德尔斯和里斯琼脂培养基上的生长和底物利用情况，所有17株菌株均被鉴定为产果胶酶和纤维素酶的根际细菌。DNS 方法的定量分析显示了不同的酶活性，突出了微生物组的生理变化。使用ASSISTAT软件对所有菌株显示的酶活性差异进行统计分析。