D-Arabitol is an important pentitol that is widely used in the food, pharmaceutical and chemical industries. It is mainly produced by yeasts during the biotransformation of glucose. To obtain strains with high D-arabitol production, Candida parapsilosis was mutated using atmospheric and room temperature plasma (ARTP). Among the screened mutants, mutant A6 had the highest yield at 32.92 g/L, a 53.98% increase compared with the original strain (21.38 g/L). Furthermore, metabolic regulators were added to the medium to improve D-arabitol production. Pyrithioxin dihydrochloride increased D-arabitol production by 34.4% by regulating glucose-6-phosphate dehydrogenase, and 4-methylpyrazole increased D-arabitol production by 77.4% compared with the control group by inhibiting alcohol dehydrogenase activity. Amphotericin B and Triton X-100 increased D-arabitol production by 23.8% and 42.2% by improving the membrane permeability and dissolved oxygen content, respectively. This study may provide important implications for obtaining high-yield D-arabitol strains.

中文翻译：

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结合诱变和代谢调节，可增强副念珠菌产生D-阿拉伯糖的能力。

D-阿拉伯糖醇是一种重要的戊糖醇，广泛用于食品，制药和化学工业。它主要由酵母在葡萄糖的生物转化过程中产生。为了获得具有高D-阿拉伯糖醇产量的菌株，使用大气和室温血浆（ARTP）使副念珠菌突变。在筛选出的突变体中，突变体A6的最高产量为32.92 g / L，比原始菌株（21.38 g / L）增加了53.98％。此外，将代谢调节剂添加到培养基中以改善D-阿拉伯糖醇的产生。通过调节葡萄糖-6-磷酸脱氢酶，吡硫酮二盐酸盐使D-阿拉伯糖醇的产生增加了34.4％，与对照组相比，4-甲基吡唑通过抑制醇脱氢酶的活性使D-阿拉伯糖的产生增加了77.4％。两性霉素B和Triton X-100通过提高膜的渗透性和溶解氧含量分别使D-阿拉伯糖醇的产量增加了23.8％和42.2％。这项研究可能为获得高产D-阿拉伯糖醇菌株提供重要的启示。