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Combined mutagenesis and metabolic regulation to enhance d-arabitol production from Candida parapsilosis

  • Biotechnology Methods - Original Paper
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

d-Arabitol is an important pentitol that is widely used in the food, pharmaceutical and chemical industries. It is mainly produced by yeasts during the biotransformation of glucose. To obtain strains with high d-arabitol production, Candida parapsilosis was mutated using atmospheric and room temperature plasma (ARTP). Among the screened mutants, mutant A6 had the highest yield at 32.92 g/L, a 53.98% increase compared with the original strain (21.38 g/L). Furthermore, metabolic regulators were added to the medium to improve d-arabitol production. Pyrithioxin dihydrochloride increased d-arabitol production by 34.4% by regulating glucose-6-phosphate dehydrogenase, and 4-methylpyrazole increased d-arabitol production by 77.4% compared with the control group by inhibiting alcohol dehydrogenase activity. Amphotericin B and Triton X-100 increased d-arabitol production by 23.8% and 42.2% by improving the membrane permeability and dissolved oxygen content, respectively. This study may provide important implications for obtaining high-yield d-arabitol strains.

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Acknowledgements

This work was supported by National Natural Science Foundation of China (31871745), National Key Research and Development Program (2017YFC1600902) and National First-Class Discipline program of Food Science and Technology (JUFSTR 20180203).

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Authors and Affiliations

  1. State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, Jiangsu, China

    Simeng Zheng, Bo Jiang, Tao Zhang & Jingjing Chen

  2. International Joint Laboratory On Food Safety, Jiangnan University, Wuxi, 214122, Jiangsu, China

    Bo Jiang, Tao Zhang & Jingjing Chen

Authors
  1. Simeng Zheng
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  2. Bo Jiang
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  3. Tao Zhang
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  4. Jingjing Chen
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Contributions

BJ and TZ helped conceive the study and BJ was in charge of overall direction and planning. SZ designed and carried out the experiments, and analyzed the data. JC contributed to the interpretation of the results. SZ wrote the manuscript with support from BJ and JC. All authors provided critical feedback and contributed to the final version of the manuscript.

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Correspondence to Bo Jiang.

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Zheng, S., Jiang, B., Zhang, T. et al. Combined mutagenesis and metabolic regulation to enhance d-arabitol production from Candida parapsilosis. J Ind Microbiol Biotechnol 47, 425–435 (2020). https://doi.org/10.1007/s10295-020-02278-4

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  • DOI: https://doi.org/10.1007/s10295-020-02278-4

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