Tomato leaf curl New Delhi virus (ToLCNDV), a single-stranded (ss)DNA virus(family; Geminiviridae), has become a constraint to the production of crops of the Cucurbitaceae, Solanaceae and Malvaceae across the sub-Continent, south east Asia and, more recently, the western Mediterranean basin. Transgenic Nicotiana benthamiana plants constitutively expressing the phage M13 gene 5 (G5) protein, an ssDNA binding protein, fused with nuclear localization signal, showed mild virus symptoms with a low virus titre. This study confirms G5 protein as useful to develop broad-spectrum resistance against ToLCNDV and other ssDNA viruses in plants.

中文翻译：

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在表达噬菌体 M13 基因 5（G5）（一种单链 DNA 结合蛋白）的转基因本氏烟草中对番茄卷叶新德里 begomovirus 的耐受性

番茄卷叶新德里病毒 (ToLCNDV) 是一种单链 (ss)DNA 病毒（家族；双生病毒科），已成为东南亚次大陆葫芦科、茄科和锦葵科作物生产的制约因素以及最近的地中海西部盆地。组成型表达噬菌体 M13 基因 5 (G5) 蛋白的转基因本氏烟草植物，一种 ssDNA 结合蛋白，与核定位信号融合，显示出轻度病毒症状，病毒滴度低。该研究证实 G5 蛋白可用于在植物中产生对 ToLCNDV 和其他 ssDNA 病毒的广谱抗性。