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Tolerance to tomato leaf curl New Delhi begomovirus in transgenic Nicotiana benthamiana expressing the phage M13 gene 5 (G5), an ssDNA binding protein

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Abstract

Tomato leaf curl New Delhi virus (ToLCNDV), a single-stranded (ss)DNA virus(family; Geminiviridae), has become a constraint to the production of crops of the Cucurbitaceae, Solanaceae and Malvaceae across the sub-Continent, south east Asia and, more recently, the western Mediterranean basin. Transgenic Nicotiana benthamiana plants constitutively expressing the phage M13 gene 5 (G5) protein, an ssDNA binding protein, fused with nuclear localization signal, showed mild virus symptoms with a low virus titre. This study confirms G5 protein as useful to develop broad-spectrum resistance against ToLCNDV and other ssDNA viruses in plants.

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Author notes

  1. Ghulam Rasool

    Present address: Plant Breeding and Genetics, Faculty of Agricultural Sciences, Ghazi University, Dera Ghazi Khan, Pakistan

  2. Sumaira Yousaf

    Present address: Nuclear Institute for Agriculture and Biology, Faisalabad, Pakistan

Authors and Affiliations

  1. National Institute for Biotechnology and Genetic Engineering, Faisalabad, Pakistan

    Ghulam Rasool, Sumaira Yousaf, Afzal Akram, Shahid Mansoor, Rob W. Briddon & Muhammad Saeed

  2. Pakistan Institute of Engineering and Applied Sciences, Islamabad, Pakistan

    Ghulam Rasool, Sumaira Yousaf & Afzal Akram

Authors
  1. Ghulam Rasool
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  2. Sumaira Yousaf
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  3. Afzal Akram
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  4. Shahid Mansoor
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  5. Rob W. Briddon
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  6. Muhammad Saeed
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Correspondence to Ghulam Rasool.

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Rasool, G., Yousaf, S., Akram, A. et al. Tolerance to tomato leaf curl New Delhi begomovirus in transgenic Nicotiana benthamiana expressing the phage M13 gene 5 (G5), an ssDNA binding protein. Trop. plant pathol. 45, 443–447 (2020). https://doi.org/10.1007/s40858-020-00342-z

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  • DOI: https://doi.org/10.1007/s40858-020-00342-z

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