Reactivation of dormant meristem in banjhi (dormant) shoots is important to enhance the quality and quantity of tea production. The field grown tea bushes were subjected to treatment with dormancy breaking agents such as potassium nitrate (KNO₃), thiourea, sodium nitro prusside (SNP), the phytohormones kinetin (Kn) and gibberellins (GA). The efficacy of Kn and GA were comparatively lesser than KNO₃ while the combination of Kn and GA (50 and100 ppm respectively) resulted in better dormancy reduction in tea buds. This observation was supported by our results from gene expression study where accumulation patterns of mRNAs corresponding to histones (H2A, H2B, H3 and H4), cyclins (B2, D1 and D3), cyclin-dependent kinase (CDKA), ubiquitination enzymes (FUS, EXT CE2), cyclophilin, E2F, and tubulin were analyzed during growth-dormancy cycles in tea apical buds under the influence of Kn, GA and their combinations. The level of these mRNAs was low in dormant buds, which was significantly increased by foliar application of GA and Kn combination. The present study indicated that the foliar application of GA in combination with Kn will help to improve quality and quantity of tea production by breaking dormancy and stimulating the bud growth.

中文翻译：

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细胞分裂素和赤霉素的协同作用刺激茶树（茶树）芽中休眠的释放。

重新激活班吉（休眠）芽中休眠的分生组织对于提高茶叶生产的质量和数量很重要。对田间种植的茶树灌木丛进行休眠破坏剂处理，例如硝酸钾（KNO ₃），硫脲，硝普鲁钠（SNP），植物激素激动素（Kn）和赤霉素（GA）。Kn和GA的疗效相对低于KNO ₃而Kn和GA的组合（分别为50和100 ppm）可以更好地减少茶芽的休眠。我们的基因表达研究结果支持了这一观察结果，其中与组蛋白（H2A，H2B，H3和H4），细胞周期蛋白（B2，D1和D3），细胞周期蛋白依赖性激酶（CDKA），泛素化酶（FUS）相对应的mRNA积累模式（EXT CE2），亲环蛋白，E2F和微管蛋白在Kn，GA及其组合的影响下在茶顶芽的生长休眠周期中进行了分析。这些mRNA的水平在休眠芽中较低，这通过叶面施用GA和Kn组合显着增加。本研究表明，将GA与Kn组合叶面施用可通过打破休眠状态和刺激芽的生长来帮助提高茶的质量和数量。