Endophytes that represent a sub-set of plant resident microbes are a reservoir of bioactive metabolites. Many of the secondary metabolite biosynthetic gene clusters of endophytes are silent under axenic culture conditions. Epigenetic reprogramming of such cryptic pathways is possible by use of small molecule modulators like prednisone. Methylation changes induced by prednisone, a hypomethylating epigenetic modulator were studied in endophytic Fusarium oxysporum. CRED–RA analysis following exposure to non-cytotoxic dose (300 µM) revealed prednisone as effective in inducing non-methylation and semi-methylation pattern while inhibiting full-methylation of the genome. Effectiveness of prednisone as a DNA methyl transferase inhibitor can be explored in future to study alterations in secondary metabolite gene expression profile in endophytic F. oxysporum.

中文翻译：

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泼尼松处理内生镰孢镰刀菌后通过CRED-RA分析评估DNA甲基化变化

代表植物驻留微生物子集的内生菌是生物活性代谢物的储库。内生菌的许多次生代谢产物生物合成基因簇在无菌培养条件下沉默。通过使用小分子调节剂（如泼尼松），可以对这种秘密途径进行表观遗传重编程。在内生尖孢镰刀菌中研究了低甲基化表观遗传调节剂强的松引起的甲基化变化。暴露于非细胞毒性剂量（300 µM）后的CRED–RA分析表明，泼尼松可有效诱导非甲基化和半甲基化模式，同时抑制基因组的全甲基化。将来可以探索泼尼松作为DNA甲基转移酶抑制剂的有效性，以研究内生F次级代谢产物基因表达谱的变化。尖孢菌。