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Evaluation of DNA Methylation Changes by CRED–RA Analysis Following Prednisone Treatment of Endophyte, Fusarium oxysporum

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Abstract

Endophytes that represent a sub-set of plant resident microbes are a reservoir of bioactive metabolites. Many of the secondary metabolite biosynthetic gene clusters of endophytes are silent under axenic culture conditions. Epigenetic reprogramming of such cryptic pathways is possible by use of small molecule modulators like prednisone. Methylation changes induced by prednisone, a hypomethylating epigenetic modulator were studied in endophytic Fusarium oxysporum. CRED–RA analysis following exposure to non-cytotoxic dose (300 µM) revealed prednisone as effective in inducing non-methylation and semi-methylation pattern while inhibiting full-methylation of the genome. Effectiveness of prednisone as a DNA methyl transferase inhibitor can be explored in future to study alterations in secondary metabolite gene expression profile in endophytic F. oxysporum.

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Acknowledgements

HK acknowledges the Grant received from UGC (University Grants Commission, Govt. of India) for the Junior Research Fellowship received (No. 20/12/2015(ii)EU-V). HK and ARN are also thankful to Department of Science and Technology (DST) for the research Grant received (EMR/2016/002229).

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  1. Department of Biochemistry and Molecular Biology, Central University of Kerala (CUK), Kasaragod, Kerala, 671 320, India

    K. Harshitha & R. Aswati Nair

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  1. K. Harshitha
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  2. R. Aswati Nair
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Correspondence to R. Aswati Nair.

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Harshitha, K., Nair, R.A. Evaluation of DNA Methylation Changes by CRED–RA Analysis Following Prednisone Treatment of Endophyte, Fusarium oxysporum. Indian J Microbiol 60, 254–258 (2020). https://doi.org/10.1007/s12088-020-00857-8

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