Background: Antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis is an autoimmune disorder of unknown etiology with dysregulated cytokines levels. Objectives: The main aim of this study was to assess the clinical correlation between antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis, granulomatosis with polyangiitis (GPA) serum levels of the microscopic polyangiitis (MPA), serum levels of the proinflammatory cytokines, interleukin (IL)-32 and interleukin-6. Methods: Study included 71 patients, 47 with GPA and 24 with MPA. Serum IL-32 and IL-6 concentrations were analyzed in all patients, and compared with levels observed in 10 controls. IL-32 and IL-6 were evaluated using DuoSet and Quantikine HS ELISA, respectively. IL-32 and IL-6 concentrations were correlated with disease-related clinical and laboratory findings. Results: IL-32 and IL-6 levels were significantly higher in GPA and MPA than in controls, especially IL-32 levels in GPA were elevated. IL-32 concentrations correlated positively with anti-proteinase 3 - ANCA (PR3-ANCA) levels in GPA (P < 0.0001), and with anti-myeloperoxidase ANCA (MPO-ANCA) in MPA(P = 0.049). IL-32 levels correlated positively with disease activity inGPA and MPA(P < 0.0001). GPA patients with pulmonary, cutaneous, and musculoskeletal involvement presented the highest IL-6 serum levels. Cutaneous manifestations correlated positively with IL-6 levels in MPA patients (P = 0.05). ANCA-positive patients with GPA expressed significantly high IL-6 levels (P = 0.036). No significant difference in IL-32 values was observed between ANCA-positiveandANCA-negativepatients. Conclusions: Patientswith GPAandMPApresenthigher serumIL-32 and IL-6 levels than controls. IL-32 levels correlate positively with disease activity.

背景：抗中性粒细胞胞浆抗体（ANCA）相关性血管炎是一种病因不明、细胞因子水平失调的自身免疫性疾病。目的：本研究的主要目的是评估抗中性粒细胞胞浆抗体（ANCA）相关性血管炎、肉芽肿性多血管炎（GPA）、显微镜下多血管炎（MPA）的血清水平、促炎细胞因子、白细胞介素的血清水平之间的临床相关性。 IL)-32和白细胞介素-6。方法：研究包括 71 名患者，其中 47 名患有 GPA，24 名患有 MPA。对所有患者的血清 IL-32 和 IL-6 浓度进行了分析，并与 10 名对照者中观察到的水平进行了比较。分别使用 DuoSet 和 Quantikine HS ELISA 评估 IL-32 和 IL-6。 IL-32 和 IL-6 浓度与疾病相关的临床和实验室检查结果相关。结果:GPA和MPA中IL-32和IL-6水平显着高于对照组,尤其是GPA中IL-32水平升高。 IL-32浓度与GPA中的抗蛋白酶3-ANCA（PR3-ANCA）水平呈正相关（P <0.0001），与MPA中的抗髓过氧化物酶ANCA（MPO-ANCA）水平呈正相关（P =0.049）。 IL-32水平与GPA和MPA疾病活动度呈正相关（P < 0.0001）。肺部、皮肤和肌肉骨骼受累的 GPA 患者血清 IL-6 水平最高。 MPA 患者的皮肤表现与 IL-6 水平呈正相关（P = 0.05）。 ANCA 阳性且 GPA 的患者表达显着较高的 IL-6 水平（P = 0.036）。 ANCA阳性和ANCA阴性患者之间IL-32值没有观察到显着差异。结论：GPA 和 MPA 患者的血清 IL-32 和 IL-6 水平高于对照组。 IL-32 水平与疾病活动度呈正相关。