Although nonwoven fabric (NWF) has been reported to be a candidate scaffold for the large-scale expansion of mesenchymal stem cells (MSCs), the quality of cells grown in NWF has not been well clarified. In this report, MSCs grown in an NWF disc for 3 weeks showed higher osteogenic differentiation potential and percentage of CD90 (+) cells than MSCs grown on the bottom surface of dish. The amount of the extracellular matrix (ECM) per unit surface area of fibers was larger than that on the bottom surface of the dish in the first 2 weeks of culture. The osteogenic differentiation potential of MSCs inoculated onto cell-free ECM increased with increasing amount of ECM. The higher percentage of CD90 (+) cells and osteogenic differentiation potential of cells grown in an NWF disc than of cells grown on a dish might, at least in part, be due to the higher amount of ECM.

中文翻译：

---

在培养皿和无纺布上培养的间充质干细胞之间CD90阳性细胞百分比和成骨分化潜能的比较。

尽管无纺布（NWF）已被报道是间充质干细胞（MSCs）大规模扩增的候选支架，但在NWF中生长的细胞的质量尚未得到很好的阐明。在此报告中，在NWF圆盘中生长3周的MSC比培养皿底部表面的MSC具有更高的成骨分化潜能和CD90（+）细胞百分比。在培养的前两周，每单位纤维表面积的细胞外基质（ECM）量大于培养皿底部表面的细胞外基质量。随着ECM量的增加，接种到无细胞ECM上的MSC的成骨分化潜能增加。NWF盘中生长的细胞比碟子上生长的细胞具有更高的CD90（+）细胞百分比和成骨分化潜能，至少部分地，