Abstract
Although nonwoven fabric (NWF) has been reported to be a candidate scaffold for the large-scale expansion of mesenchymal stem cells (MSCs), the quality of cells grown in NWF has not been well clarified. In this report, MSCs grown in an NWF disc for 3 weeks showed higher osteogenic differentiation potential and percentage of CD90 (+) cells than MSCs grown on the bottom surface of dish. The amount of the extracellular matrix (ECM) per unit surface area of fibers was larger than that on the bottom surface of the dish in the first 2 weeks of culture. The osteogenic differentiation potential of MSCs inoculated onto cell-free ECM increased with increasing amount of ECM. The higher percentage of CD90 (+) cells and osteogenic differentiation potential of cells grown in an NWF disc than of cells grown on a dish might, at least in part, be due to the higher amount of ECM.
Similar content being viewed by others
References
Anasiz Y, Ozgul RK, Uckan-Cetinkaya D (2017) A new chapter for mesenchymal stem cells: decellularized extracellular matrices. Stem Cell Rev. 13:587–597
Bonab MM, Alimoghaddam K, Talebian F, Ghaffari SH, Ghavamzadeh A, Nikbin B (2006) Aging of mesenchymal stem cell in vitro. BMC Cell Biol 7:14
Chen XD, Dusevich V, Feng JQ, Manolagas SC, Jilka RL (2007) Extracellular matrix made by bone marrow cell facilitates expansion of marrow-derived mesenchymal progenitor cell and prevents their differentiation into osteoblasts. J Bone Miner Res 22:1943–1956
Engler AJ, Sen S, Sweeney HL, Discher DE (2006) Matrix elasticity directs stem cell lineage specification. Cell 126:677–689
Fu B, Yamada K, Fujiwara M, Iwai R, Takagi M (2019) Protocol of mesenchymal stem cell inoculation to nonwoven fabric scaffold. Cytotechnology 71:743–750
Hoch AI, Leach JK (2014) Concise review: optimizing expansion of bone marrow mesenchymal stem/stromal cells for clinical applications. Stem Cells Transl Med 3:643
Lai Y, Sun Y, Skinner CM, Son EL, Lu Z, Tuan RS, Jilka RL, Ling J, Chen XD (2010) Reconstitution of marrow-derived extracellular matrix ex vivo: a robust culture system for expanding large-scale highly functional human mesenchymal stem cells. Stem Cells Dev 19:1095–1107
Leu S, Lin YC, Yuen CM, Yen CH, Kao YH, Sun CK, Yip HK (2010) Adipose-derived mesenchymal stem cells markedly attenuate brain infarct size and improve neurological function in rats. J Transl Med 8:63
Moraes DA, Sibov TT, Pavon LF, Alvim PQ, Bonadio RS, Da Silva JR, Pic-Taylor A, Toledo OA, Marti LC, Azevedo RB, Oliveira DM (2016) A reduction in CD90 (THY-1) expression results in increased differentiation of mesenchymal stromal cells. Stem Cell Res Ther 7:97
Pandolfi L, Furman NT, Wang X, Lupo C, Martinez JO, Mohamed M, Taraballi F, Tasciotti E (2017) A nanofibrous electrospun patch to maintain human mesenchymal cell stemness. J Mater Sci Mater Med 28:44
Rakian R, Block TJ, Johnson SM, Marinkovic M, Wu J, Dai Q, Dean DD, Chen XD (2015) Native extracellular matrix preserves mesenchymal stem cell “stemness” and differentiation potential under serum-free culture conditions. Stem Cell Res Ther 6:235
Røsland GV, Svendsen A, Torsvik A, Sobala E, McCormack E, Immervoll H, Mysliwietz J, Tonn JC, Goldbrunner R, Lønning PE, Bjerkvig R, Schichor C (2009) Long-term cultures of bone marrow-derived human mesenchymal stem cells frequently undergo spontaneous malignant transformation. Cancer Res 69:5331–5339
Sanford KK, Earle WR, Evans VJ, Waltz HK, Shannon JE (1950) The measurement of proliferation in tissue cultures by enumeration of cell nuclei. Anat Rec 106:243
Sasaki T, Takagi M, Soma T, Yoshida T (2003) Analysis of hematopoietic microenvironment containing spatial development of stromal cells in nonwoven fabrics. J Biosci Bioeng 96:76–78
Sato Y, Wakitani S, Takagi M (2013) Xeno-free and shrinkage-free preparation of scaffold-free cartilage-like disc-shaped cell sheet using human bone marrow mesenchymal stem cells. J Biosci Bioeng 116:734–739
Schellenberg A, Ross R, Abagnale G, Joussen S, Schuster P, Arshi A, Pallua N, Jockenhoevel S, Gries T, Wagner W (2014) 3D non-woven polyvinylidene fluoride scaffolds: fibre cross section and texturizing patterns have impact on growth of mesenchymal stromal cells. PLoS ONE 9:e94353
Sekiya I, Larson BL, Smith JR, Pochampally R, Cui JG, Prockop DJ (2002) Expansion of human adult stem cells from bone marrow stroma: conditions that maximize the yields of early progenitors and evaluate their quality. Stem Cells. 20:530–541
Sudhakararao P, Jessica M, Thomas K (2014) Decellularized ECM effects on human mesenchymal stem cell stemness and differentiation. Differentiation 88:131–143
Turoverova LV, Khotin MG, Iudintseva NM, Magnusson KE, Blinova MI, Pinaev GP, Tentler DG (2009) Analysis of extracellular matrix proteins produced by cultured cells. Tsitologiia 51:691–696
Xu Y, Shi T, Xu A, Zhang L (2016) 3D spheroid culture enhances survival and therapeutic capacities of MSCs injected into ischemic kidney. J Cell Mol Med 20:1203–1213
Yamahara K, Taguchi A, Soma T, Ogawa H, Ikeda T, Yoshimatsu J (2014) Clinical application of human amnion-derived mesenchymal stem cells for the treatment of acute GVHD. Placenta 35:A6
Author information
Authors and Affiliations
Corresponding author
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Fu, B., Fujiwara, M. & Takagi, M. Comparison of percentage of CD90-positive cells and osteogenic differentiation potential between mesenchymal stem cells grown on dish and nonwoven fabric. Cytotechnology 72, 433–444 (2020). https://doi.org/10.1007/s10616-020-00390-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10616-020-00390-y