Production of 2,3-butanediol (2,3-BD) by Pantoea agglomerans strain BL1 was investigated using soybean hull hydrolysate as substrate in batch reactors. The cultivation media consisted of a mixture of xylose, arabinose, and glucose, obtained from the hemicellulosic fraction of the soybean hull biomass. We evaluated the influence of oxygen supply, pH control, and media supplementation on the growth kinetics of the microorganism and on 2,3-BD production. P. agglomerans BL1 was able to simultaneously metabolize all three monosaccharides present in the broth, with average conversions of 75% after 48 h of cultivation. The influence of aeration conditions employed demonstrated the mixed acid pathway of 2,3-BD formation by enterobacteria. Under fully aerated conditions (2 vvm of air), up to 14.02 g L-1 of 2.3-BD in 12 h of cultivation were produced, corresponding to yields of 0.53 g g-1 and a productivity of 1.17 g L-1 h-1, the best results achieved. These results suggest the production potential of 2,3-BD by P. agglomerans BL1, which has been recently isolated from an environmental consortium. The present work proposes a solution for the usage of the hemicellulosic fraction of agroindustry biomasses, carbohydrates whose utilization are not commonly addressed in bioprocess.

中文翻译：

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成团泛菌使用大豆皮酸水解产物作为底物在生物反应器中生产 2,3-丁二醇。


使用大豆皮水解产物作为底物在间歇式反应器中研究成团泛菌菌株 BL1 生产 2,3-丁二醇 (2,3-BD)。培养基由从大豆皮生物质的半纤维素部分获得的木糖、阿拉伯糖和葡萄糖的混合物组成。我们评估了供氧、pH 控制和培养基补充对微生物生长动力学和 2,3-BD 生产的影响。 P. agglomerans BL1 能够同时代谢肉汤中存在的所有三种单糖，培养 48 小时后平均转化率为 75%。通气条件的影响证明了肠细菌形成 2,3-BD 的混合酸途径。在完全通气条件下（2 vvm 空气），在 12 小时的培养中产生了高达 14.02 g L-1 的 2.3-BD，相当于 0.53 g g-1 的产量和 1.17 g L-1 h-的生产率。 1、取得最佳效果。这些结果表明 P. agglomerans BL1 具有生产 2,3-BD 的潜力，该细菌最近从环境联盟中分离出来。目前的工作提出了一种利用农工业生物质的半纤维素部分的解决方案，碳水化合物的利用在生物过程中通常不被解决。