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Bioreactor production of 2,3-butanediol by Pantoea agglomerans using soybean hull acid hydrolysate as substrate

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Abstract

Production of 2,3-butanediol (2,3-BD) by Pantoea agglomerans strain BL1 was investigated using soybean hull hydrolysate as substrate in batch reactors. The cultivation media consisted of a mixture of xylose, arabinose, and glucose, obtained from the hemicellulosic fraction of the soybean hull biomass. We evaluated the influence of oxygen supply, pH control, and media supplementation on the growth kinetics of the microorganism and on 2,3-BD production. P. agglomerans BL1 was able to simultaneously metabolize all three monosaccharides present in the broth, with average conversions of 75% after 48 h of cultivation. The influence of aeration conditions employed demonstrated the mixed acid pathway of 2,3-BD formation by enterobacteria. Under fully aerated conditions (2 vvm of air), up to 14.02 g L−1 of 2.3-BD in 12 h of cultivation were produced, corresponding to yields of 0.53 g g−1 and a productivity of 1.17 g L−1 h−1, the best results achieved. These results suggest the production potential of 2,3-BD by P. agglomerans BL1, which has been recently isolated from an environmental consortium. The present work proposes a solution for the usage of the hemicellulosic fraction of agroindustry biomasses, carbohydrates whose utilization are not commonly addressed in bioprocess.

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Acknowledgements

The authors wish to thank Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação do Aperfeiçoamento de Pessoal do Ensino Superior (CAPES), Finance Code 001, and Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS) for their financial support of this project and scholarships.

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  1. Biotechnology and Biochemical Engineering Laboratory (BiotecLab), Federal University of Rio Grande do Sul, Porto Alegre, Brazil

    Laura Jensen Ourique, Camille Conte Rocha, Raul Charpinel Diniz Gomes & Marco Antônio Záchia Ayub

  2. Department of Chemical Engineering, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil

    Daniele Misturini Rossi

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Ourique, L.J., Rocha, C.C., Gomes, R.C.D. et al. Bioreactor production of 2,3-butanediol by Pantoea agglomerans using soybean hull acid hydrolysate as substrate. Bioprocess Biosyst Eng 43, 1689–1701 (2020). https://doi.org/10.1007/s00449-020-02362-0

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