当前位置: X-MOL 学术Folia Microbiol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Effect of the anti-inflammatory drug diclofenac on lipid composition of bacterial strain Raoultella sp. KDF8.
Folia Microbiologica ( IF 2.4 ) Pub Date : 2020-04-21 , DOI: 10.1007/s12223-020-00790-9
Andrea Palyzová 1 , Helena Marešová 1 , Jiří Novák 1 , Jiří Zahradník 1 , Tomáš Řezanka 1
Affiliation  

The strain Raoultella sp. KDF8 was cultivated on three sources of carbon and energy, glycerol, ethanol and diclofenac, for periods of time ranging from 24 to 72 h. Using thin-layer chromatography, nine classes of phospholipids were detected and the amount of phosphatidylethanolamine (PtdEtn) decreased with increasing cultivation time. Conversely, the ratio of phospholipids having three or four acyls (acyl-phosphatidylglycerol (APtdGro), N-acyl-PtdEtn (NAPtdEtn) and cardiolipin (Ptd2Gro) increased during cultivation. GC-MS analysis showed that the percentage of fatty acids containing a cyclopropane ring increased almost tenfold whereas the amount of fatty acids bearing even-numbered chains dropped to less than one-third after 24 h and 72 h in cultures on glycerol and diclofenac, respectively. Shotgun analysis showed significant changes in the representation of molecular species of phospholipids. For instance, there was a 36-fold change in the ratio of 16:1/16:1/16:1-APtdGro to c17:0/c17:0/c17:0-APtdGro and a 12-fold ratio change for 16:1/16:1/16:1-NAPtdEtn to c17:0/c17:0/c17:0-NAPtdEtn; the Ptd2Gro ratio of 16:1 to c17:0 acids equalled 1750. Our results show that the bacteria overcome destabilization of the inner cytoplasmic cell membrane and a bacterial outer membrane by altering the geometric arrangement of acyl chains, i.e. switching from monounsaturated to cyclopropane fatty acids (16:1 versus c17:0).

中文翻译:

抗炎药双氯芬酸对细菌劳拉氏菌属菌株脂质组成的影响。KDF8。

劳拉氏菌菌株。KDF8在碳和能源的三种来源(甘油,乙醇和双氯芬酸)上培养了24至72小时。使用薄层色谱法,检测到九类磷脂,并且随着培养时间的增加,磷脂酰乙醇胺(PtdEtn)的数量减少。相反,在培养过程中,具有三个或四个酰基(酰基-磷脂酰甘油(APtdGro),N-酰基-PtdEtn(NAPtdEtn)和心磷脂(Ptd2Gro))的磷脂比例增加; GC-MS分析表明,含有环丙烷的脂肪酸的百分比在甘油和双氯芬酸上分别培养24小时和72小时后,环的数量几乎增加了十倍,而带有偶数链的脂肪酸的量分别降至不到三分之一。gun弹枪分析显示磷脂分子种类的表示形式发生了显着变化。例如,16:1/16:1/16:1-APtdGro与c17:0 / c17:0 / c17:0-APtdGro的比率发生了36倍的变化,而16的比率发生了12倍的变化:1/16:1/16:1-NAPtdEtn到c17:0 / c17:0 / c17:0-NAPtdEtn; Ptd2Gro 16:1与c17:0酸的比率等于1750。我们的结果表明,细菌可以通过改变酰基链的几何排列来克服细胞质内膜和细菌外膜的不稳定,即从单不饱和脂肪转变为环丙烷脂肪酸(16:1对c17:0)。0-NAPtdEtn;Ptd2Gro 16:1与c17:0酸的比率等于1750。我们的结果表明,细菌可以通过改变酰基链的几何排列来克服细胞质内膜和细菌外膜的不稳定,即从单不饱和脂肪转变为环丙烷脂肪酸(16:1对c17:0)。0-NAPtdEtn; Ptd2Gro 16:1与c17:0酸的比率等于1750。我们的结果表明,细菌可以通过改变酰基链的几何排列来克服细胞质内膜和细菌外膜的不稳定,即从单不饱和脂肪转变为环丙烷脂肪酸(16:1对c17:0)。
更新日期:2020-04-21
down
wechat
bug