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Effect of the anti-inflammatory drug diclofenac on lipid composition of bacterial strain Raoultella sp. KDF8

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Abstract

The strain Raoultella sp. KDF8 was cultivated on three sources of carbon and energy, glycerol, ethanol and diclofenac, for periods of time ranging from 24 to 72 h. Using thin-layer chromatography, nine classes of phospholipids were detected and the amount of phosphatidylethanolamine (PtdEtn) decreased with increasing cultivation time. Conversely, the ratio of phospholipids having three or four acyls (acyl-phosphatidylglycerol (APtdGro), N-acyl-PtdEtn (NAPtdEtn) and cardiolipin (Ptd2Gro) increased during cultivation. GC-MS analysis showed that the percentage of fatty acids containing a cyclopropane ring increased almost tenfold whereas the amount of fatty acids bearing even-numbered chains dropped to less than one-third after 24 h and 72 h in cultures on glycerol and diclofenac, respectively. Shotgun analysis showed significant changes in the representation of molecular species of phospholipids. For instance, there was a 36-fold change in the ratio of 16:1/16:1/16:1-APtdGro to c17:0/c17:0/c17:0-APtdGro and a 12-fold ratio change for 16:1/16:1/16:1-NAPtdEtn to c17:0/c17:0/c17:0-NAPtdEtn; the Ptd2Gro ratio of 16:1 to c17:0 acids equalled 1750. Our results show that the bacteria overcome destabilization of the inner cytoplasmic cell membrane and a bacterial outer membrane by altering the geometric arrangement of acyl chains, i.e. switching from monounsaturated to cyclopropane fatty acids (16:1 versus c17:0).

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Acknowledgments

This research was supported by the Ministry of Education, Youth and Sports of the Czech Republic (LO1509) and the Epsilon programme of the Technology Agency of the Czech Republic (TH02030337).

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Authors and Affiliations

  1. Institute of Microbiology, Czech Academy of Sciences, Vídeňská 1083, 142 20, Prague 4, Czech Republic

    Andrea Palyzová, Helena Marešová, Jiří Novák, Jiří Zahradník & Tomáš Řezanka

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  1. Andrea Palyzová
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  2. Helena Marešová
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Correspondence to Tomáš Řezanka.

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Palyzová, A., Marešová, H., Novák, J. et al. Effect of the anti-inflammatory drug diclofenac on lipid composition of bacterial strain Raoultella sp. KDF8. Folia Microbiol 65, 763–773 (2020). https://doi.org/10.1007/s12223-020-00790-9

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