Shock waves are used to treat musculoskeletal injuries and trigger the body’s mechanisms to initiate healing; however, the cellular and molecular working mechanisms are not fully known. Raman spectroscopy may be a useful tool to provide information on structural changes. Solid collagen type I from rat tail (> 90% pure) was suspended in water and was exposed in vitro to different numbers of shock waves and energy flux densities. Raman spectra were recorded at 2 h, 1 week, and 3 weeks after shock-wave treatment. The spectral analysis indicated that varying the number of shock waves and the energy flux density induced molecular changes in the collagen structure. Varying the energy flux density induced more significant changes than modifying the number of shock waves; however, in most cases, the collagen recovered its original conformation 3 weeks after treatment. A significant decrease in the relative intensities of the conformational bands, which include amide I, amide III, and stretching C–C, was observed at different energy flux densities. In many clinical cases, the natural repair of tissue is improved after shock-wave treatment. Raman spectroscopy revealed that varying the energy flux density of the shock waves applied to rat collagen type I induced strong conformational molecular changes. Approximately 2–3 weeks after shock-wave treatment, a phase of “molecular ordering” tending to a “recovering molecular sequence repair” was observed.

中文翻译：

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冲击波次数和能量通量密度对大鼠I型胶原拉曼光谱的影响

冲击波用于治疗肌肉骨骼损伤并触发身体的机制以启动愈合；然而，细胞和分子的工作机制尚不完全清楚。拉曼光谱可能是提供结构变化信息的有用工具。来自鼠尾的固体 I 型胶原蛋白（纯度 > 90%）悬浮在水中，并在体外暴露于不同数量的冲击波和能量通量密度。在冲击波治疗后 2 小时、1 周和 3 周记录拉曼光谱。光谱分析表明，改变冲击波的数量和能量通量密度会引起胶原结构中的分子变化。改变能量通量密度引起的变化比改变冲击波的数量更显着；然而，在大多数情况下，胶原蛋白在治疗后 3 周恢复其原始构象。在不同的能量通量密度下观察到构象带的相对强度显着降低，包括酰胺 I、酰胺 III 和拉伸 C-C。在许多临床病例中，冲击波治疗后组织的自然修复得到改善。拉曼光谱显示，改变施加于大鼠 I 型胶原蛋白的冲击波的能量通量密度会引起强烈的分子构象变化。冲击波治疗后大约 2-3 周，观察到一个“分子排序”阶段，趋向于“恢复分子序列修复”。在不同的能量通量密度下观察到。在许多临床病例中，冲击波治疗后组织的自然修复得到改善。拉曼光谱显示，改变施加于大鼠 I 型胶原蛋白的冲击波的能量通量密度会引起强烈的分子构象变化。冲击波治疗后大约 2-3 周，观察到一个“分子排序”阶段，趋向于“恢复分子序列修复”。在不同的能量通量密度下观察到。在许多临床病例中，冲击波治疗后组织的自然修复得到改善。拉曼光谱显示，改变施加于大鼠 I 型胶原蛋白的冲击波的能量通量密度会引起强烈的分子构象变化。冲击波治疗后大约 2-3 周，观察到一个“分子排序”阶段，趋向于“恢复分子序列修复”。