Calycosin-7-O-β-D-glucoside (CG), a methoxylated isoflavonoid in Astragalus membranaceus Fisch. (Bunge), has a wide range of biological activities. Adventitious root culture is an alternative source to obtain active compounds. To improve CG production in A. membranaceus adventitious roots (AMARs), effect of nitrogen concentration, and phosphate concentration on accumulation of biomass, CG, and hydrogen peroxide were investigated. A low concentration of nitrogen promoted growth and CG accumulation in AMARs. Maximum biomass and CG productivity were obtained at a nitrogen concentration of 3 mM. Nitrate, rather than ammonium, was suitable for CG production. Phosphate concentration did not influence biomass accumulation; however, high concentration of phosphate (2.5 mM) was most favorable for CG accumulation. Furthermore, moderate levels of hydrogen peroxide promoted CG accumulation. Optimal concentrations of nitrogen and phosphate were used in scale-up culture of AMARs using a bioreactor. There was a 8- and 2- fold increase in the CG content and antioxidant activity, respectively, using a B5 medium containing 3 mM NO₃⁻ and 2.5 mM phosphate when compared to the standard B5 medium, which was equivalent to those observed in field-grown plants. Therefore, optimization of nitrogen and phosphate concentration in the medium is a simple and effective method to economically improve production of CG in AMARs.

Calycosin-7-O-β-D-葡萄糖苷（CG），黄芪Fisch中的甲氧基化异黄酮。（蹦极），具有广泛的生物活性。不定根培养是获得活性化合物的替代来源。改善膜A. CG的生产研究了不定根（AMARs），氮浓度和磷酸盐浓度对生物量，CG和过氧化氢积累的影响。氮的低浓度促进了AMARs的生长和CG积累。在3 mM的氮浓度下获得了最大的生物量和CG生产率。硝酸盐而非铵盐适合于CG生产。磷酸盐浓度不影响生物量的积累；但是，高浓度的磷酸盐（2.5 mM）最有利于CG的积累。此外，适量的过氧化氢促进了CG的积累。使用生物反应器在AMAR的按比例放大培养中使用了最佳浓度的氮和磷酸盐。使用含有3 mM NO的B5培养基，其CG含量和抗氧化活性分别增加了8倍和2倍。₃ ^-和2.5mM磷酸盐相比时标准B5培养基，相当于与在田间生长的植物中观察到。因此，优化培养基中氮和磷酸盐的浓度是一种经济有效地提高AMAR中CG产量的简单有效的方法。