INTRODUCTION Plants respond to changes in their environments through hormonal activation of a physiological cascade that redirects metabolic resources and growth. In filberts (Corylus sp.), chelated iron promotes the growth of new shoots but the mechanism(s) are not understood. OBJECTIVES To use untargeted metabolomics and hormonomics approaches to generate novel hypotheses for the morphoregulatory role of ferric ethylenediamine-N,N'-di-(ortho-hydroxyphenyl) acetic acid (Fe-EDDHA) in filbert shoot organogenesis in vitro. METHODS Data were generated using previously optimized standardized untargeted metabolomics protocols with time of flight mass spectrometry. Multivariate statistical tools (principal component and partial least squares discriminant analysis) did not detect significant differences. Discovery tools Significance Analysis of Microarrays (SAM), multiple linear regression analysis, Bayesian analysis, logical algorithms, machine learning, synthetic biotransformations, targeted hormonomics, and online resources including MetaboAnalyst were used. RESULTS Starch/sucrose metabolism and shikimate pathway metabolites were increased. Dose dependent decreases were found in polyphenol metabolism, specifically ellagic acid and its methylated derivative 3,4,3'-tri-O-methylellagic acid. Hormonomics analysis revealed significant differences in phytohormones and their conjugates. FeEDDHA treatment reduced indole-3-acetic acid, abscisic acid, salicylic acid, jasmonic acid conjugates (JA-Trp, JA-Ile, OH-JA) and dihydrozeatinglucoside in regenerating explants. Serotonin (5HT) was decreased in FeEDDHA-treated regenerating tissues while the related metabolite melatonin was increased. Eight phenolic conjugates of 5HT and eight catabolites were affected by FeEDDHA indicating that metabolism to sequester, deactivate and metabolize 5HT was induced by Fe(III). Tryptophan was metabolized through kynurenine but not anthranilate. CONCLUSION Seven novel hypotheses were generated to guide future studies to understand the regulatory control(s) of shoot organogenesis.

中文翻译：

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代谢组学和人机工程学可以破解欧洲榛树生长的代码。

简介植物通过激素激活重新分配代谢资源和生长的生理级联反应来响应环境变化。在欧洲榛树（Corylus sp。）中，螯合的铁促进新芽的生长，但其机理尚不清楚。目的使用非目标代谢组学和人机工程学方法，为乙二胺-N，N'-二-（邻羟基苯基）乙酸铁（Fe-EDDHA）在欧洲榛树芽器官体外的形态调控作用中产生新的假设。方法使用飞行时间质谱，使用先前优化的标准化非靶向代谢组学方案生成数据。多元统计工具（主要成分和偏最小二乘判别分析）未发现显着差异。使用了发现工具微阵列意义分析（SAM），多元线性回归分析，贝叶斯分析，逻辑算法，机器学习，合成生物转化，靶向的人机工程学以及包括MetaboAnalyst在内的在线资源。结果淀粉/蔗糖代谢和sh草酸酯途径代谢产物增加。在多酚代谢中发现剂量依赖性降低，特别是鞣花酸及其甲基化衍生物3,4,3'-三-O-甲基鞣花酸。人体工程学分析显示植物激素及其结合物存在显着差异。FeEDDHA处理可减少再生外植体中的吲哚-3-乙酸，脱落酸，水杨酸，茉莉酸结合物（JA-Trp，JA-Ile，OH-JA）和二氢玉米素葡糖苷。在FeEDDHA处理的再生组织中，血清素（5HT）降低，而相关代谢产物褪黑激素增加。FeEDDHA影响了8HT的8种酚类共轭物和8种分解代谢物，表明Fe（III）诱导了5HT的螯合，失活和代谢。色氨酸通过犬尿氨酸代谢，但不通过邻氨基苯甲酸代谢。结论产生了七个新颖的假设，以指导未来的研究，以了解芽器官发生的调控。