Biocides of antifouling agents can cause problems in marine ecosystems by damaging to non-target algal species. Aquatic bioassays are important means of assessing the quality of water containing mixtures of contaminants and of providing a safety standard for water management in an ecological context. In this study, a rapid, sensitive and inexpensive test method was developed using free-living male and female gametophytes of the brown macroalga Undaria pinnatifida. A conventional fluorometer was employed to evaluate the acute (48 h) toxic effects of six antifouling biocides: 4,5-Dichloro-2-octyl-isothiazolone (DCOIT), diuron, irgarol, medetomidine, tolylfluanid, zinc pyrithione (ZnPT). The decreasing toxicity in male and female gametophytes as estimated by EC50 (effective concentration at which 50% inhibition occurs) values was: diuron (0.037 and 0.128 mg l-1, respectively) > irgarol (0.096 and 0.172 mg l-1, respectively) > tolylfluanid (0.238 and 1.028 mg l-1, respectively) > DCOIT (1.015 and 0.890 mg l-1, respectively) > medetomidine (12.032 and 12.763 mg l-1, respectively). For ZnPT, 50% fluorescence inhibition of U. pinnatifida gametophytes occurred at concentrations above 0.4 mg l-1. The Undaria method is rapid, simple, practical, and cost-effective for the detection of photosynthesis-inhibiting biocides, thus making a useful tool for testing the toxicity of antifouling agents in marine environments.

中文翻译：

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使用基于微孔板的叶绿素荧光对裙带菜配子体中的六种防污增强杀菌剂进行快速毒性评估。


防污剂的杀菌剂可能会损害非目标藻类物种，从而给海洋生态系统带来问题。水生生物测定是评估含有污染物混合物的水的质量并为生态环境中的水管理提供安全标准的重要手段。在这项研究中，利用棕色大型藻裙带菜的自由生活的雄性和雌性配子体开发了一种快速、灵敏且廉价的测试方法。采用传统荧光计评估了六种防污杀菌剂的急性（48小时）毒性作用：4,5-二氯-2-辛基异噻唑酮（DCOIT）、敌草隆、伊加罗、美托咪定、甲苯氟苯胺、吡啶硫酮锌（ZnPT）。根据 EC50（发生 50% 抑制的有效浓度）值估计，雄性和雌性配子体的毒性降低为：敌草隆（分别为 0.037 和 0.128 mg l-1）> irgarol（分别为 0.096 和 0.172 mg l-1） > 甲苯氟苯哌啶（分别为 0.238 和 1.028 mg l-1） > DCOIT（分别为 1.015 和 0.890 mg l-1） > 美托咪定（分别为 12.032 和 12.763 mg l-1）。对于 ZnPT，浓度高于 0.4 mg l-1 时，U. pinnatifida 配子体的荧光抑制发生 50%。裙带菜方法快速、简单、实用且经济高效，可用于检测光合作用抑制杀菌剂，从而成为测试海洋环境中防污剂毒性的有用工具。