This study investigated stimulatory potentials of zero-charged calcium nanoparticles (CaNPs) on nutritional contents and free radical scavenging abilities of Moringa oleifera. M. oleifera seeds were planted on soil wetted with water (control: group A), 100 mgL⁻¹ Ca(NO₃)₂, 100, 75 and 50 mgL⁻¹ CaNPs as groups B, C, D and E respectively. Elemental compositions were determined with inductively coupled plasma optical emission spectrometer (ICP-OES). CaNPs beneficially improved crude fibre, ash, K and Ca by 124.82, 152.5, 10.66 and 28.91% respectively in M. oleifera. There were significant declines in concentrations of Na, Ni, Pb, Cd and Cr in M. oleifera planted on CaNPs amended soil. CaNPs significantly improved free radical scavenging ability from 43.85 to 74.06%, phenolic contents from 248.82 to 555.88 mg quercetin/g and flavonoid contents from 120.75 to 148.08 mg quercetin/g. Reduction in oxidative stress biomarker (MDA) from 1.83 to 1.54 mmolg⁻¹ in M. oleifera planted on CaNPs amended soil was also recorded. Strong correlations among essential mineral pairs indicate M. oleifera is a source of multiple mineral nutrients and a poor source of toxic heavy metals. Results of estimated daily intake of mineral nutrients and toxic hazard quotients of heavy metals suggest M. oleifera planted on CaNPs as the safest for consumption with respect to heavy metal toxicity. Lifetime risk assessment reveals a 50% reduction in cancer risk from consumption of M. oleifera planted on CaNPs soil. Thus, it is evidently clear from results of this study that CaNPs enhanced nutritional quality of M. oleifera.

中文翻译：

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纳米钙（CaNP）对辣木营养品质、自由基清除特性的影响以及对人类健康的风险评估

本研究调查了零电荷钙纳米粒子（CaNP）对辣木营养成分和自由基清除能力的刺激潜力。将油橄榄种子种植在用水润湿的土壤上（对照：A组），100 mgL ^-1 Ca(NO ₃ ) ₂、100、75和50 mgL ^-1 CaNP分别作为B、C、D和E组。元素组成用电感耦合等离子体发射光谱仪（ICP-OES）测定。CaNPs 有益地改善了油橄榄的粗纤维、灰分、钾和钙，分别提高了 124.82%、152.5%、10.66% 和 28.91% 。种植在 CaNPs 改良土壤上的油橄榄中 Na、Ni、Pb、Cd 和 Cr 的浓度显着下降。CaNPs显着提高了自由基清除能力，从43.85％提高到74.06％，酚类含量从248.82毫克槲皮素/克提高到555.88毫克槲皮素/克，黄酮类化合物含量从120.75毫克槲皮素/克提高到148.08毫克槲皮素/克。还记录了种植在 CaNPs 改良土壤上的油橄榄的氧化应激生物标志物 (MDA) 从 1.83 至 1.54 mmolg ^-1减少。必需矿物质对之间的强相关性表明，油橄榄是多种矿物质营养素的来源，也是有毒重金属的不良来源。估计每日矿物质营养素摄入量和重金属毒性危险商的结果表明，就重金属毒性而言，种植在 CaNP 上的油橄榄是最安全的食用油。终生风险评估显示，食用种植在 CaNPs 土壤上的油橄榄可使癌症风险降低 50%。因此，从本研究结果可以明显看出，CaNPs 提高了油橄榄的营养质量。