Engineering polyketide biosynthesis to enable the production of diverse chemical structures is a major challenge at present. Utilising an established biosynthetic cassette for terminal alkyne production, Porterfield et al. applied docking domain and site-directed mutagenesis approaches to interface these enzymes with modular polyketide synthase (PKS) enzymes, yielding products with a bio-orthogonal handle.

中文翻译：

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单击入位：将末端炔烃生物合成与聚酮化合物合酶连接。

目前，工程化聚酮化合物的生物合成以产生多种化学结构是一项重大挑战。Porterfield等人利用已建立的生物合成盒用于末端炔烃生产。应用对接结构域和定点诱变方法将这些酶与模块化聚酮化合物合酶（PKS）酶连接，产生具有生物正交柄的产物。