Trends in Biotechnology
Volume 38, Issue 7, July 2020, Pages 682-684
SpotlightClicking into Place: Interfacing Terminal Alkyne Biosynthesis with Polyketide Synthases
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Cited by (1)
Probing the structure and function of acyl carrier proteins to unlock the strategic redesign of type II polyketide biosynthetic pathways
2021, Journal of Biological ChemistryCitation Excerpt :Harnessing the terminal alkyne biosynthetic machinery from the jamaicamide B type I PKS pathway enabled access to in trans loading of alkyne polyketides onto noncognate type I PKS enzymes. This process was optimized through the strategic modification of the jamaicamide B ACP via docking domain installation and site-directed mutagenesis (107, 108). The selective, copper-catalyzed reaction of the terminal alkyne with an azide-containing fluorophore or mass tag can potentially enable subsequent visualization via fluorescence or mass spectrometry.
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