In this research paper we filter and verify miRNAs which may target silent information regulator homolog 2 (SIRT2) gene and then describe the mechanism whereby miRNA-212 might regulate lipogenic genes in mammary epithelial cell lines via targeting SIRT2. Bioinformatics analysis revealed that the bovine SIRT2 gene is regulated by three miRNAs: miR-212, miR-375 and miR-655. The three miRNAs were verified and screened by qRT-PCR, western blot, and luciferase multiplex verification techniques and only miR-212 was shown to have a targeting relationship with SIRT2. The results of co-transfecting miR-212 and silencing RNA (siRNA) showed that by targeting SIRT2, miR-212 can regulate the expression of fatty acid synthetase (FASN) and sterol regulatory element binding factor 1 (SREBP1) but not peroxisome proliferator-activated receptor gamma (PPARγ). Measurement of triglyceride (TAG) content showed that miR-212 increased the fat content of mammary epithelial cell lines. The study indicates that miR-212 could target and inhibit the expression of the SIRT2 gene to promote lipogenesis in mammary epithelial cell lines.

中文翻译：

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MicroRNA-212 靶向 SIRT2 影响牛乳腺上皮细胞系的脂肪生成

在这篇研究论文中，我们过滤和验证了可能靶向沉默信息调节器同源物 2 的 miRNA（SIRT2) 基因，然后描述其中的机制miRNA-212可能调节乳腺上皮细胞系中的脂肪生成基因通过定位SIRT2. 生物信息学分析表明，牛SIRT2基因受三种 miRNA 调控：miR-212,miR-375和miR-655. 这三种 miRNA 通过 qRT-PCR、蛋白质印迹和荧光素酶多重验证技术进行了验证和筛选，并且仅miR-212被证明与SIRT2。共转染结果miR-212和沉默 RNA (siRNA) 表明，通过靶向SIRT2,miR-212可以调节脂肪酸合成酶的表达（FASN) 和甾醇调节元件结合因子 1 (SREBP1) 但不是过氧化物酶体增殖物激活受体 γ (PPARγ）。甘油三酯 (TAG) 含量的测量表明，miR-212增加乳腺上皮细胞系的脂肪含量。研究表明，miR-212可以靶向并抑制SIRT2促进乳腺上皮细胞系中脂肪生成的基因。