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MicroRNA-212 targets SIRT2 to influence lipogenesis in bovine mammary epithelial cell line

Published online by Cambridge University Press:  16 April 2020

Xubin Lu ,
Hailei Xia ,
Jingyi Jiang ,
Xin Xu ,
Mingxun Li ,
Zhi Chen ,
Yujia Sun ,
Huimin Zhang  and
Zhangping Yang
Xubin Lu
Affiliation:
College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu225002, China
Hailei Xia
Affiliation:
College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu225002, China
Jingyi Jiang
Affiliation:
College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu225002, China
Xin Xu
Affiliation:
College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu225002, China
Mingxun Li
Affiliation:
College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu225002, China
Zhi Chen
Affiliation:
College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu225002, China
Yujia Sun
Affiliation:
Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou University, Yangzhou225009, Jiangsu Province, China
Huimin Zhang
Affiliation:
College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu225002, China
Zhangping Yang*
Affiliation:
College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu225002, China
*
Author for correspondence: Zhangping Yang, Email: yzp@yzu.edu.cn
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Abstract

In this research paper we filter and verify miRNAs which may target silent information regulator homolog 2 (SIRT2) gene and then describe the mechanism whereby miRNA-212 might regulate lipogenic genes in mammary epithelial cell lines via targeting SIRT2. Bioinformatics analysis revealed that the bovine SIRT2 gene is regulated by three miRNAs: miR-212, miR-375 and miR-655. The three miRNAs were verified and screened by qRT-PCR, western blot, and luciferase multiplex verification techniques and only miR-212 was shown to have a targeting relationship with SIRT2. The results of co-transfecting miR-212 and silencing RNA (siRNA) showed that by targeting SIRT2, miR-212 can regulate the expression of fatty acid synthetase (FASN) and sterol regulatory element binding factor 1 (SREBP1) but not peroxisome proliferator-activated receptor gamma (PPARγ). Measurement of triglyceride (TAG) content showed that miR-212 increased the fat content of mammary epithelial cell lines. The study indicates that miR-212 could target and inhibit the expression of the SIRT2 gene to promote lipogenesis in mammary epithelial cell lines.

Keywords

LipogenesismiR-212miR-375miR-655SIRT2
Type
Research Article
Information
Journal of Dairy Research , Volume 87 , Issue 2 , May 2020 , pp. 232 - 238
Copyright
Copyright © Hannah Dairy Research Foundation 2020

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