The use of several lignocellulosic and other organic wastes has been considered in literature for the production of L-asparaginases (L-ASNase); however, the use of olive mill wastewater (OMWW) has never been contemplated as culture media. OMWW is a widely available waste in the Mediterranean that needs to be treated due to its high pollutant load. In this work, OMWW is used to feed the strain Bacillus aryabhattai Ba03, which reduces the content in phenolic compounds and produces L-ASNase. A water dilution of OMWW to 25% (w/w) supplemented with 10 g/L of yeast extract at pH 8, incubated with the strain at 30 °C, was suitable for the production of 11.6 ± 0.3 U/mL extracellular L-ASNase after 2 days. In addition, the decrease of phenolic compounds together with the production of L-ASNase could be associated with the generation of aspartic acid and the expression of peroxidase activity. At the end of the bioprocess, salicylic acid was identified in the culture supernatant, assuming that chorismate metabolic pathway was followed by B. aryabhattai Ba03.

在文献中已经考虑使用几种木质纤维素和其他有机废物来生产L-天冬酰胺酶（L-ASNase）。但是，从未考虑将橄榄磨废水（OMWW）用作培养基。OMWW是地中海地区广泛使用的废物，由于其高污染物负荷，需要对其进行处理。在这项工作中，OMWW被用于喂养aryabhattai芽孢杆菌BaO 3降低酚类化合物的含量并产生L-ASNase。将OMWW的水稀释液稀释至25％（w / w），并补充10 g / L的pH 8酵母提取物，并在30°C下与该菌株温育，适用于生产11.6±0.3 U / mL的细胞外L- 2天后ASNase。此外，酚类化合物的减少以及L-ASNase的产生可能与天冬氨酸的产生和过氧化物酶活性的表达有关。在生物过程结束时，假定分支酸盐的代谢途径是跟随阿里巴发芽孢杆菌Ba03 ，则在培养上清液中鉴定出水杨酸。