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Valorization of olive mill wastewater towards the production of L-asparaginases

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Abstract

The use of several lignocellulosic and other organic wastes has been considered in literature for the production of L-asparaginases (L-ASNase); however, the use of olive mill wastewater (OMWW) has never been contemplated as culture media. OMWW is a widely available waste in the Mediterranean that needs to be treated due to its high pollutant load. In this work, OMWW is used to feed the strain Bacillus aryabhattai Ba03, which reduces the content in phenolic compounds and produces L-ASNase. A water dilution of OMWW to 25% (w/w) supplemented with 10 g/L of yeast extract at pH 8, incubated with the strain at 30 °C, was suitable for the production of 11.6 ± 0.3 U/mL extracellular L-ASNase after 2 days. In addition, the decrease of phenolic compounds together with the production of L-ASNase could be associated with the generation of aspartic acid and the expression of peroxidase activity. At the end of the bioprocess, salicylic acid was identified in the culture supernatant, assuming that chorismate metabolic pathway was followed by B. aryabhattai Ba03.

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Acknowledgments

We are grateful to Professor George Zervakis for providing the olive mill wastewater, and to the “Consellería de Cultura, Educación e Ordenación Universitaria” of Xunta de Galicia (Spain) for the postdoctoral fellowship of Alicia Pérez Paz (ED481B2018/073). Professor Nikolaos Thomaidis and his research group TrAMS are acknowledged for providing the LC-MS analysis of olive mill wastewater.

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Authors and Affiliations

  1. Industrial Biotechnology & Biocatalysis Group, Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 5 Iroon Polytechniou Str., Zografou Campus, 15780, Athens, Greece

    Alicia Paz, Efstratios Nikolaivits & Evangelos Topakas

  2. Industrial Biotechnology and Environmental Engineering Group “BiotecnIA”, Chemical Engineering Department, University of Vigo (Campus Ourense), As Lagoas s/n, 32004, Ourense, Galicia, Spain

    Alicia Paz

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  1. Alicia Paz
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Paz, A., Nikolaivits, E. & Topakas, E. Valorization of olive mill wastewater towards the production of L-asparaginases. Biomass Conv. Bioref. 11, 539–546 (2021). https://doi.org/10.1007/s13399-020-00725-x

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  • DOI: https://doi.org/10.1007/s13399-020-00725-x

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