Serine catabolism produces ROS, sensitizes cells to actin dysfunction, and suppresses cell growth in fission yeast.,The Journal of Antibiotics

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Serine catabolism produces ROS, sensitizes cells to actin dysfunction, and suppresses cell growth in fission yeast.
The Journal of Antibiotics ( IF 2.1 ) Pub Date : 2020-04-20 , DOI: 10.1038/s41429-020-0305-6
Akihiko Kanou ₁ , Shinichi Nishimura _{1,

2,

3,

4} , Toshitsugu Tabuchi ₂ , Akihisa Matsuyama _{2,

4} , Minoru Yoshida _{2,

3,

4} , Taira Kato ₅ , Hideaki Kakeya ₁

Affiliation

Serine is an essential component in organisms as a building block of biomolecules, a precursor of metabolites, an allosteric regulator of an enzyme, etc. This amino acid is thought to be a key metabolite in human diseases including cancers and infectious diseases. To understand the consequence of serine catabolism, we screened natural products to identify a fungal metabolite chaetoglobosin D (ChD) as a specific inhibitor of fission yeast cell growth when cultivated with serine as a sole nitrogen source. ChD targets actin, and actin mutant cells showed severe growth defect on serine medium. ROS accumulated in cells when cultivated in serine medium, while actin mutant cells showed increased sensitivity to oxidative stress. ROS production is a new aspect of serine metabolism, which might be involved in disease progression, and actin could be the drug target for curing serine-dependent symptoms.

中文翻译：

丝氨酸分解代谢产生ROS，使细胞对肌动蛋白功能异常敏感，并抑制裂变酵母中的细胞生长。

丝氨酸是生物分子中必不可少的组成部分，是生物分子的组成部分，代谢产物的前体，酶的变构调节剂等。这种氨基酸被认为是人类疾病（包括癌症和传染病）中的关键代谢产物。为了了解丝氨酸分解代谢的结果，我们筛选了天然产物，以鉴定当以丝氨酸为唯一氮源培养时，真菌代谢产物chaetoglobosin D（ChD）是裂变酵母细胞生长的特异性抑制剂。ChD靶向肌动蛋白，肌动蛋白突变细胞在丝氨酸培养基上显示出严重的生长缺陷。当在丝氨酸培养基中培养时，ROS积累在细胞中，而肌动蛋白突变细胞则显示出对氧化应激的增强敏感性。ROS的产生是丝氨酸代谢的一个新方面，可能与疾病进展有关，

更新日期：2020-04-24

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