Abstract

The biogenic synthesis of metal nanoparticles (MNPs) is gaining impetus and widely used for being an eco-friendly approach. In this study, bacterium Pseudomonas stutzeri was demonstrated for use in the biogenic synthesis of gold nanoparticles (AuNPs). Optimization of conditions indicated that 3 mM chloroauric acid (HAuCl₄), pH 9, incubation at 80 °C for 60 min, etc. were optimum for AuNPs synthesis. AuNPs were then synthesized with these optimum conditions and characterized using various spectroscopic and imaging techniques. The initial screening of 22 different metal ions for colloidal AuNPs based visual and UV–Vis. spectra based sensing indicated that two metal ion namely Manganese (Mn²⁺) and Platinum (Pt²⁺) caused both, the change in color from ruby red to dark brown and purple-blue along with spectral shifts of 23 and 21 nm respectively, therefore, could be easily detected. The test of sensitivity indicated that 100 and 190 ppm of Mn²⁺ and Pt²⁺ respectively were the limit of detections and, the assay quantitatively recovered Mn²⁺ and Pt²⁺ in the range of 70–150% with considerable accuracy and precision.

中文翻译：

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细菌介导的简便绿色方法优化金纳米颗粒的生物合成，可简单直观地检测两种金属离子

摘要

金属纳米颗粒（MNP）的生物合成正在获得动力，并被广泛用作一种生态友好的方法。在这项研究中，证明了斯氏假单胞菌可用于金纳米颗粒（AuNPs）的生物合成。条件的优化表明，3 mM的氯金酸（HAuCl ₄），pH 9，在80°C下温育60分钟等对于AuNPs合成是最佳的。然后在这些最佳条件下合成AuNP，并使用各种光谱和成像技术对其进行表征。初步筛选基于胶体金纳米粒子的22种不同金属离子的视觉和UV-Vis。基于光谱的传感表明，两种金属离子即锰（Mn ²⁺）和铂（Pt ²⁺）引起了这两种情况，从红宝石红到深棕色和紫蓝色的颜色变化以及分别为23和21 nm的光谱偏移，因此可以很容易地检测到。灵敏度测试表明，Mn ²⁺和Pt ²⁺分别为100和190 ppm是检测的极限，该测定法定量回收的Mn ²⁺和Pt ²⁺在70–150％的范围内，具有相当高的准确性和精密度。 。