Leaf abscission of rose (Rosa hybrida L. ‘Baby Love’) cultured in vitro due to ethylene gas accumulation and enzymatic hydrolytic activity, is one of the abnormal phenomena affecting the shoots quality. In this study, silver nanoparticles (AgNPs) and cobalt nanoparticles (CoNPs) were used to overcome leaves abscission as well as, the effect of shoot mass propagation, rooting and acclimatization at the nursery stage. The results showed that shoots cultured on MS (Murashige and Skoog, in Physiol Plantarum 15(3):473–497, 10.1111/j.1399-3054.1962.tb08052.x, 1962) medium supplemented with 2 mg/L AgNPs gave the highest shoot multiplication coefficient, shoot height, fresh weight, dry weight and chlorophyll index (5.33 shoots; 3.06 cm; 451.00 mg; 58.33 mg; 32.28; respectively) than CoNPs (replace CoCl₂ in the MS medium) and basal MS medium. Meanwhile in the rooting stage, MS medium added of 4.65 µg/L CoNPs was the most optimal with dry mass ratio (10.28%), number of roots (5.67 roots), root length (2.17 cm) and SPAD (41.07 nmol/cm²) as well as reduced the ethylene gas content (0.11 ppm) and enzymes activity such as pectinase (0.07 UI/mL) and cellulase (0.25 UI/mL) in comparision to the other treatments after 4 weeks culture. The plantlets derived from in vitro culture on medium added 4.65 µg/L CoNPs gave the highest survival rate (96.67%) as well as growth and development at the nursery stage.

由于乙烯气体的积累和酶水解活性，离体培养的玫瑰（Rosa hybrida L.'Baby Love'）的叶片脱落是影响枝条品质的异常现象之一。在这项研究中，银纳米颗粒（AgNPs）和钴纳米颗粒（CoNPs）用于克服叶片脱落以及苗期苗芽繁殖，生根和驯化的影响。结果表明，在补充有2 mg / L AgNPs的MS（Murashige和Skoog，在Physiol Plantarum 15（3）：473-497，10.1111 / j.1399-3054.1962.tb08052.x，1962）上培养的芽最高。枝条繁殖系数，枝条高度，鲜重，干重和叶绿素指数（分别为5.33条； 3.06 cm； 451.00 mg； 58.33 mg； 32.28；）比CoNPs（取代CoCl _2）在MS培养基中）和基础MS培养基中。同时，在生根阶段，添加4.65 µg / L CoNPs的MS培养基最理想，其干质量比（10.28％），根数（5.67根），根长（2.17 cm）和SPAD（41.07 nmol / cm ^2）培养4周后，与其他处理相比，降低了乙烯气体含量（0.11 ppm）和酶活性，如果胶酶（0.07 UI / mL）和纤维素酶（0.25 UI / mL）。在培养基上添加4.65 µg / L CoNPs进行体外培养后得到的小植株，在育苗期的成活率最高，达96.67％。