Abstract
Leaf abscission of rose (Rosa hybrida L. ‘Baby Love’) cultured in vitro due to ethylene gas accumulation and enzymatic hydrolytic activity, is one of the abnormal phenomena affecting the shoots quality. In this study, silver nanoparticles (AgNPs) and cobalt nanoparticles (CoNPs) were used to overcome leaves abscission as well as, the effect of shoot mass propagation, rooting and acclimatization at the nursery stage. The results showed that shoots cultured on MS (Murashige and Skoog, in Physiol Plantarum 15(3):473–497, 10.1111/j.1399-3054.1962.tb08052.x, 1962) medium supplemented with 2 mg/L AgNPs gave the highest shoot multiplication coefficient, shoot height, fresh weight, dry weight and chlorophyll index (5.33 shoots; 3.06 cm; 451.00 mg; 58.33 mg; 32.28; respectively) than CoNPs (replace CoCl2 in the MS medium) and basal MS medium. Meanwhile in the rooting stage, MS medium added of 4.65 µg/L CoNPs was the most optimal with dry mass ratio (10.28%), number of roots (5.67 roots), root length (2.17 cm) and SPAD (41.07 nmol/cm2) as well as reduced the ethylene gas content (0.11 ppm) and enzymes activity such as pectinase (0.07 UI/mL) and cellulase (0.25 UI/mL) in comparision to the other treatments after 4 weeks culture. The plantlets derived from in vitro culture on medium added 4.65 µg/L CoNPs gave the highest survival rate (96.67%) as well as growth and development at the nursery stage.
Key message
Cobalt and silver nanoparticles (CoNPs, AgNPs) were used to overcome leaf abscission, enhance growth and increase the survival rate of plantlets at the nursery stage. CoNPs helps reduce the ethylene gas content, enzymatic hydrolytic activity such as pectinase and cellulase. AgNPs was the most suitable factor for shoots mass propagation.
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Abbreviations
- AgNPs:
-
Silver nanoparticles
- CoNPs:
-
Cobalt nanoparticles
- MS:
-
Murashige and Skoog
- MS-CoNPs:
-
CoNPs was used to replace CoCl2
- MS-AgNPs:
-
Supplemented AgNPs in to the culture medium
- CoCl2 :
-
Cobalt chloride
- SPAD:
-
Chlorophyll content in leaf
- AZ:
-
Abscission zone
- ACC-l:
-
Aminocyclopropane-1-carboxylic acid
- DNS:
-
3,5-Dinitrosalicylic acid
- CMC:
-
Carboxymethyl cellulose
- GC:
-
Gas chromatography
- BA-6:
-
Benzylaminopurine
- NAA-α:
-
Naphthaleneacetic acid
- IBA-3:
-
Indolebutyric acid
- DM:
-
Dry mass ratio
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This work was supported by the National Foundation for Science and Technology Development (NAFOSTED), Vietnam under Project Code 106.01-2019.301.
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Ngan Thi My Ha acquired data wrote the manuscript. Tung Thanh Hoang, Cuong Do Manh, Nghiep Dai Ngo, Le Van Bui participated in interpretation of data and revision for intellectual content. Duong Tan Nhut conceptualized and designed the study. All authors discussed the results and contributed to the final manuscript.
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Ha, N.T.M., Manh Do, C., Hoang, T.T. et al. The effect of cobalt and silver nanoparticles on overcoming leaf abscission and enhanced growth of rose (Rosa hybrida L. ‘Baby Love’) plantlets cultured in vitro. Plant Cell Tiss Organ Cult 141, 393–405 (2020). https://doi.org/10.1007/s11240-020-01796-4
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DOI: https://doi.org/10.1007/s11240-020-01796-4