The expression analysis of GhNAC4, a NAC (NAM, ATAF1-2, and CUC2) domain-containing transcription factor of the upland cotton, Gossypium hirsutum was carried out. Its expression was up-regulated by abscisic acid, cytokinin, methyl jasmonic acid, gibberellic acid, auxin, and ethylene. Its expression was also highly induced by drought, osmotic, oxidative, salinity, high and low-temperature stresses, and wounding. To corroborate these observations, we cloned the promoter of GhNAC4 and fused it transcriptionally with uidA (GUS) gene for studies in transgenic tobacco. Fluorometric GUS analysis of the transgenic plants revealed that it is also induced by various phytohormones and environmental stresses. The spatio-temporal analysis of the GhNAC4 promoter revealed that GUS expression was active in all stages of plant development including the reproductive organs. In the mature plant, the GhNAC4 is expressed at a higher level in vascular bundles and guard cells. We also observed intense expression in other cells upon wounding. These observations were supported by a detailed bioinformatic analysis of the GhNAC4 promoter for identifying the cis-acting elements that are associated with the regulation of gene expression in a tissue-specific and induced manner.

的表达分析GhNAC4，一个NAC（NAM，ATAF1 - 2，和CUC2）陆地棉的结构域的转录因子，陆地棉进行。其表达被脱落酸，细胞分裂素，甲基茉莉酸，赤霉素，生长素和乙烯上调。干旱，渗透，氧化，盐分，高温和低温胁迫以及伤口也高度诱导了其表达。为了证实这些发现，我们克隆了GhNAC4的启动子，并将其与uidA转录融合。（GUS）基因用于转基因烟草研究。对转基因植物的荧光GUS分析表明，它也受到多种植物激素和环境胁迫的诱导。GhNAC4启动子的时空分析表明，GUS表达在植物发育的所有阶段（包括生殖器官）均活跃。在成熟植物中，GhNAC4在血管束和保卫细胞中以较高的水平表达。我们还观察到受伤后其他细胞中的强烈表达。这些观察结果得到了GhNAC4启动子的详细生物信息学分析的支持，以鉴定顺式与以组织特异性和诱导方式调节基因表达有关的作用元件。