This study reports a new detection process for sensing ultra-low concentrations of tetracycline (TC), using a hot spot surface-enhanced Raman scattering (SERS) sensor. A gold nanoparticles/ macroporous silicon (Au NPs/macroPSi) hot spot SERS sensor was fabricated using a very simple and low cost method. The SERS signal was investigated using Au NPs/macroPSi hot spot SERS sensor for efficient detection of TC antibiotics at lower concentrations of (10 −3 –10 −9 ) mol/L. The sensor showed an excellent performance for TC detection with an enhancement factor (EF) of 2 × 10 8 , ultra-low detection limit of 10 −9 mol/L, and very high reproducibility with a relative standard deviation of 2%. The effect of the pH value on the behavior of the SERS spectra for TC antibiotic was evaluated, and it was found that pH values of 5 and 6 were the best for the detection process of TC antibiotic.

中文翻译：

---

开发高效纳米生化传感器检测水中超低浓度四环素抗生素残留的新途径

本研究报告了一种使用热点表面增强拉曼散射 (SERS) 传感器检测超低浓度四环素 (TC) 的新检测过程。金纳米粒子/大孔硅（Au NPs/macroPSi）热点SERS传感器是使用非常简单和低成本的方法制造的。使用 Au NPs/macroPSi 热点 SERS 传感器研究 SERS 信号，以有效检测 (10 -3 –10 -9 ) mol/L 较低浓度的 TC 抗生素。该传感器表现出优异的TC检测性能，增强因子（EF）为2×10 8 ，超低检测限为10 -9 mol/L，重现性非常高，相对标准偏差为2%。评估了 pH 值对 TC 抗生素的 SERS 光谱行为的影响，