Neisseria gonorrhoeae, an obligatory human pathogen causes the sexually transmitted disease gonorrhea, which remains a global health problem. N. gonorrhoeae primarily infects the mucosa of the genitourinary tract, which in women, is colonized by natural microbiota, dominated by Lactobacillus spp., that protect human cells against pathogens. In this study, we demonstrated that precolonization of human epithelial cells with Lactobacillus crispatus, one of the most prevalent bacteria in the female urogenital tract, or preincubation with the L. crispatus enolase or glutamine synthetase impairs the adhesion and invasiveness of N. gonorrhoeae toward epithelial cells, two crucial steps in gonococcal pathogenesis. Furthermore, decreased expression of genes encoding the proinflam-matory cytokines, TNFα and CCL20, which are secreted as a consequence of N. gonorrhoeae infection, was observed in N. gonorrhoeae-infected epithelial cells that had been preco-lonized with L. crispatus or preincubated with enolase and glutamine synthetase. Thus, our results indicate that the protection of human cells against N. gonorrhoeae infection is a complex process and that L. crispatus and its proteins enolase and glutamine synthetase can have a potential role in protecting epithelial cells against gonococcal infection. Therefore, these results are important since disturbances of the micro-biota or of its proteins can result in dysbiosis, which is associated with increased susceptibility of epithelium to pathogens.

中文翻译：

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松脆乳杆菌及其烯醇酶和谷氨酰胺合成酶影响淋病奈瑟氏球菌与人上皮细胞之间的相互作用。

淋病奈瑟氏球菌是人类的一种强制性病原体，它导致性传播疾病淋病，这仍然是一个全球性的健康问题。淋病奈瑟氏球菌主要感染泌尿生殖道的粘膜，在女性中，它被天然微生物菌群定居，该菌群以乳杆菌属为主，可以保护人类细胞免受病原体侵害。在这项研究中，我们展示了人类上皮细胞的那precolonization卷曲乳杆菌，在女性生殖道最常见的细菌之一，或预孵化与卷曲乳杆菌烯醇化酶或谷氨酰胺合成削弱了黏附和侵袭淋球菌上皮细胞，淋球菌发病机理中的两个关键步骤。此外，编码proinflam-matory细胞因子，TNFα和CCL20，其被分泌为的结果的基因的表达降低淋病奈瑟氏球菌感染，观察到淋病奈瑟氏球菌感染的已被PRECO-lonized与上皮细胞卷曲乳杆菌或用烯醇酶和谷氨酰胺合成酶预孵育。因此，我们的结果表明，保护人类细胞免受淋病奈瑟氏球菌感染是一个复杂的过程，而crispatus其蛋白烯醇酶和谷氨酰胺合成酶在保护上皮细胞免受淋球菌感染方面可能具有潜在作用。因此，这些结果很重要，因为微生物群或其蛋白质的紊乱会导致营养不良，这与上皮对病原体的敏感性增加有关。