Eggs are one of the most common foods in the world, and their safety is a major concern for the public. We aimed to develop a novel clean-up approach named Bond Elut Enhanced Matrix Removal-Lipid (EMR-Lipid) for the simultaneous determination of 325 pesticides in the chicken egg by UHPLC–MS/MS and GC–MS/MS. The initial step of extraction was adopted by acetonitrile with 5% formic acid and subsequent adoption of EMR-Lipid d-SPE for further clean-up. Compared with the traditional C18 procedure, EMR-Lipid clean-up achieved a superior degreasing effect. The recoveries of at least 85% of pesticides were in the range of 60–120% at three fortified levels and the relative standard deviation of nearly 96% of analytes were within the limits of criteria. Improved linearity was evaluated using a matrix-matched calibration at eight concentrations in the range 0.1–40 µg/kg; the correlation coefficients of 309 analytes were exceeding 0.99. The new method offered quick, reliable and consistent detection and quantification of 325 pesticide residues, thereby demonstrating promising future for sample testing.

中文翻译：

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UHPLC-MS/MS 和 GC-MS/MS 使用 EMR-Lipid 净化测定鸡蛋中 325 种农药的多残留

鸡蛋是世界上最常见的食物之一，其安全性是公众关注的主要问题。我们旨在开发一种名为 Bond Elut Enhanced Matrix Removal-Lipid (EMR-Lipid) 的新型净化方法，用于通过 UHPLC-MS/MS 和 GC-MS/MS 同时测定鸡蛋中的 325 种农药。提取的初始步骤采用含 5% 甲酸的乙腈，随后采用 EMR-Lipid d-SPE 进行进一步净化。与传统的C18程序相比，EMR-Lipid净化达到了优越的脱脂效果。在三个强化水平下，至少 85% 的农药的回收率在 60-120% 的范围内，近 96% 的分析物的相对标准偏差在标准范围内。使用基质匹配校准在 0.1–40 µg/kg 范围内的八个浓度下评估线性度的提高；309 种分析物的相关系数超过 0.99。新方法提供了对 325 种农药残留的快速、可靠和一致的检测和定量，从而展示了样品检测的广阔前景。