Strain KSNA2^T, a Gram-negative, moderately halophilic, facultatively anaerobic, motile, rod-shaped bacterium, was isolated from the surface-sterilized stem tissue of a beach morning glory (Cafystegia soldanella) plant in Chuja Island, Jeju-do, Republic of Korea. Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences revealed that strain KSNA2^T formed a distinct lineage within the family Enterobacteriaceae, with the highest 16S rRNA gene sequence similarity to Izhakiella australiensis KCTC 72143 (96.2%) and Izhakiella capsodis KCTC 72142^T (96.0%), exhibited 95.5–95.9% similarity to other genera in the family Enterobacter-iaceae and Erwiniaceae. Conserved signature indels analysis elucidated that strain KSNA2^T was delimited into family Enterobacteriaceae. KSNA2 genome comprises a circular chromosome of 5,182,800 bp with 56.1% G + C content Digital DNA-DNA relatedness levels between strain KSNA2^T and 18 closely related species were 19.3 to 21.1%. Average nucleotide identity values were between 72.0 and 76.7%. Growth of strain KSNA2^T was observed at 4 to 45°C (optimum, 25°C) and pH 5.0 to 12.0 (optimum, pH 7.0) in the presence of 0 to 11% (w/v) NaCl (optimum, 0–7%). The major cellular fatty acids (> 10%) were C₁₆:o followed by summed feature 8 (C₁₈ω7c and/or C₁₈ω6c), summed feature 3 (C_16:1 ω7c and/or C_16:1 ω6c), C_17:0cyclo, and C_14:0. The major isoprenoid quinone was ubiquinone-8 (Q-8). With combined phylogenetic, genomic, phenotypic, and chemotaxo-nomic features, strain KSNA2^T represents a novel species of a new genus in the family Enterobacteriaceae, for which the name Jejubacter calystegiae gen. nov., sp. nov. is proposed. The type strain is KSNA2^T (= KCTC 72234^T = CCTCC AB 2019098^T).

中文翻译：

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凯氏杆菌基因。十一月，sp。十一月，中等嗜盐性，是肠杆菌科的新成员，与海滩牵牛花隔离。

从位于共和国济州岛丘加岛的海滩牵牛花（Cafystegia soldanella）植物的表面灭菌茎组织中分离出KSNA2 ^T菌株，该菌株为革兰氏阴性，中度嗜盐，兼性厌氧，能运动的杆状细菌。韩国。根据16S rRNA基因和全基因组序列的系统进化分析表明，KSNA2 ^T菌株在肠杆菌科中形成了独特的谱系，与澳大利亚伊热克氏菌KCTC 72143（96.2％）和卡扎伊热氏菌KCTC 72142 ^T（ 96.0％），与肠杆菌科的其他属有95.5–95.9％的相似性和欧文藻科。保守标签插入缺失分析阐明了应变KSNA2 ^ŧ被分隔成家庭肠杆菌。KSNA2基因组包含一个5,182,800 bp的圆形染色体，其G + C含量为56.1％。菌株KSNA2 ^T与18个紧密相关物种之间的数字DNA-DNA相关性水平为19.3％至21.1％。平均核苷酸同一性值在72.0至76.7％之间。在0至11％（w / v）的NaCl（最佳，0-v）下，在4至45°C（最佳，25°C）和pH 5.0至12.0（最佳，pH 7.0）下观察到KSNA2 ^T菌株的生长。7％）。主要细胞脂肪酸（> 10％）为C ₁₆：○随后求和特征8（C ₁₈ ω7 Ç和/或C ₁₈ ω 6c）中，总结特征3（C _16：1个ω 7C和/或C _16:1 ω6 c）中，C _17：0环，和C _14：0。主要的类异戊二烯醌是泛醌8（Q-8）。具有组合的系统发育，基因组，表型和化学分类学特征，KSNA2 ^T菌株代表肠杆菌科中一种新属的新种，其名称为Jejubacter calystegiae gen。十一月，sp。十一月 被提议。类型菌株是KSNA2 ^T（= KCTC 72234 ^T = CCTCC AB 2019098 ^T）。