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Jejubacter calystegiae gen. nov., sp. nov., moderately halophilic, a new member of the family Enterobacteriaceae, isolated from beach morning glory

  • Microbial Systematics and Evolutionary Microbiology
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Abstract

Strain KSNA2T, a Gram-negative, moderately halophilic, facultatively anaerobic, motile, rod-shaped bacterium, was isolated from the surface-sterilized stem tissue of a beach morning glory (Cafystegia soldanella) plant in Chuja Island, Jeju-do, Republic of Korea. Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences revealed that strain KSNA2T formed a distinct lineage within the family Enterobacteriaceae, with the highest 16S rRNA gene sequence similarity to Izhakiella australiensis KCTC 72143 (96.2%) and Izhakiella capsodis KCTC 72142T (96.0%), exhibited 95.5–95.9% similarity to other genera in the family Enterobacter-iaceae and Erwiniaceae. Conserved signature indels analysis elucidated that strain KSNA2T was delimited into family Enterobacteriaceae. KSNA2 genome comprises a circular chromosome of 5,182,800 bp with 56.1% G + C content Digital DNA-DNA relatedness levels between strain KSNA2T and 18 closely related species were 19.3 to 21.1%. Average nucleotide identity values were between 72.0 and 76.7%. Growth of strain KSNA2T was observed at 4 to 45°C (optimum, 25°C) and pH 5.0 to 12.0 (optimum, pH 7.0) in the presence of 0 to 11% (w/v) NaCl (optimum, 0–7%). The major cellular fatty acids (> 10%) were C16:o followed by summed feature 8 (C18ω7c and/or C18ω6c), summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), C17:0cyclo, and C14:0. The major isoprenoid quinone was ubiquinone-8 (Q-8). With combined phylogenetic, genomic, phenotypic, and chemotaxo-nomic features, strain KSNA2T represents a novel species of a new genus in the family Enterobacteriaceae, for which the name Jejubacter calystegiae gen. nov., sp. nov. is proposed. The type strain is KSNA2T (= KCTC 72234T = CCTCC AB 2019098T).

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Acknowledgments

We thank Dr. Aharon Oren for his assistance with the nomenclature and etymology. We also thank Dr. Belinda C. Ferrari for providing the full-length sequence of the 16S rRNA gene of I. australiensis KCTC 72143T. This work was carried out with support from the KRIBB Research Initiative Program (KGM5281913).

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Authors and Affiliations

  1. Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup, 56212, Republic of Korea

    Lingmin Jiang, Jung-Sook Lee, Jae Cheol Jeong, Cha Young Kim, Suk Weon Kim & Jiyoung Lee

  2. Department of Bioactive Materials, Jeonbuk National University, Jeonju, 54896, Republic of Korea

    Lingmin Jiang, Dexin Wang & Dae-Hyuk Kim

  3. Radiation Utilization and facilities Management Division, Korea Atomic Energy Research Institute, jeongeup, 56212, Republic of Korea

    Dexin Wang

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  1. Lingmin Jiang
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Jiang, L., Wang, D., Lee, JS. et al. Jejubacter calystegiae gen. nov., sp. nov., moderately halophilic, a new member of the family Enterobacteriaceae, isolated from beach morning glory. J Microbiol. 58, 357–366 (2020). https://doi.org/10.1007/s12275-020-9294-1

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