Large animal models have been widely used to facilitate the translation of mesenchymal stem cells (MSC) from the laboratory to patient. MSC, with their multi-potent capacity, have been proposed to have therapeutic benefits in a number of pathological conditions. Laboratory studies allow the investigation of cellular and molecular interactions, while small animal models allow initial ‘proof of concept’ experiments. Large animals (dogs, pigs, sheep, goats and horses) are more similar physiologically and structurally to man. These models have allowed clinically relevant assessments of safety, efficacy and dosing of different MSC sources prior to clinical trials. In this review, we recapitulate the use of large animal models to facilitate the use of MSC to treat myocardial infarction—an example of one large animal model being considered the ‘gold standard’ for research and osteoarthritis—an example of the complexities of using different large animal models in a multifactorial disease. These examples show how large animals can provide a research platform that can be used to evaluate the value of cell-based therapies and facilitate the process of ‘bench to bedside’.

中文翻译：

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使用大型动物促进间充质干细胞从实验室到患者的过程——“工作台到床边”。

大型动物模型已被广泛用于促进间充质干细胞（MSC）从实验室到患者的转化。MSC 具有多效能力，已被认为对许多病理状况具有治疗作用。实验室研究可以研究细胞和分子相互作用，而小动物模型可以进行初步的“概念验证”实验。大型动物（狗、猪、绵羊、山羊和马）在生理和结构上与人类更相似。这些模型允许在临床试验之前对不同 MSC 来源的安全性、有效性和剂量进行临床相关评估。在这篇综述中，我们概述了大型动物模型的使用，以促进使用 MSC 治疗心肌梗塞——一个大型动物模型被认为是研究和骨关节炎“黄金标准”的例子——一个使用不同方法的复杂性的例子多因素疾病的大型动物模型。这些例子表明大型动物如何提供一个研究平台，可用于评估基于细胞的疗法的价值并促进“从实验室到临床”的过程。