Endophytic fungi can produce beneficial active components during symbiosis with host plants. Fifteen purified endophytic fungal strains were isolated and screened from root, stem, leaf, and fruit of the Chinese medicine food homologous plant Siraitia grosvenorii. These strains were fermented, and then the fermented solution was extracted using acetonitrile. The extracts were analyzed by high performance liquid chromatography (HPLC) and mass spectrum (MS), using standard mogroside V as control. The results showed that strain LHG-F5 and strain LHG-L4 could produce mogroside V with output of 3.814 µg/mL and 2.675 µg/mL, respectively. By morphological observation together with similarity of internal transcribed spacer (ITS) sequence by Clustan X method, LHG-F5 and LHG-L4 were classified as Diaporthe angelica and Fusarium solani, respectively.

中文翻译：

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从罗汉果中分离的产罗汉果苷 V 的内生真菌

内生真菌在与寄主植物共生过程中可以产生有益的活性成分。从中药食同源植物罗汉果的根、茎、叶和果实中分离筛选出15株纯化的内生真菌菌株。将这些菌株发酵，然后使用乙腈提取发酵液。提取物通过高效液相色谱 (HPLC) 和质谱 (MS) 进行分析，使用标准罗汉果苷 V 作为对照。结果表明，菌株 LHG-F5 和菌株 LHG-L4 可生产罗汉果苷 V，产量分别为 3.814 µg/mL 和 2.675 µg/mL。通过形态学观察和Clustan X方法的内部转录间隔（ITS）序列的相似性，LHG-F5和LHG-L4分别被归类为Diaporthe angelica和Fusarium solani。